The objective of this experiment in Lab #2 was to identify an unknown substance using different chemical tests to determine what organic molecules it contains. These tests are referred to as the Benedict’s, Lugol’s, Biuret, and Sudan IV tests and are each used to identify an organic compound. For instance, Benedict’s checks for reducing sugars, Lugol’s for starch, Biuret for protein, and Sudan IV for lipids. When testing the unknown with each reagent, the substance only tested positive for reducing sugars. Based on the results of this experiment and what was known about the possible unknowns it could have been, it was concluded that the unknown substance was apple juice.
Introduction
The objective of this lab is to learn and understand how to utilize chemical tests to determine the organic compounds a substance contains. As explained in “The Molecular Composition of Cells,” the …show more content…
Presence Absence Absence Absence Absence
Discussion Because the unknown tested positive for reducing sugars and negative for the rest of the organic compounds, these results coincide with my hypothesis. From these results, my conclusion is that the unknown is apple juice as apple juice has an abundance of sugar, but not of starch, lipids, or protein. In regard to errors made during the experiment, while the quantity of each substance was measured to be a balanced amount for both the unknown and the reagents, it is possible that the solution was not mixed thorough enough to receive a proper reaction. Waiting an appropriate amount of time for the right reaction to occur may also have a cause for error in the
Introduction
The objective of this lab is to learn and understand how to utilize chemical tests to determine the organic compounds a substance contains. As explained in “The Molecular Composition of Cells,” the …show more content…
Presence Absence Absence Absence Absence
Discussion Because the unknown tested positive for reducing sugars and negative for the rest of the organic compounds, these results coincide with my hypothesis. From these results, my conclusion is that the unknown is apple juice as apple juice has an abundance of sugar, but not of starch, lipids, or protein. In regard to errors made during the experiment, while the quantity of each substance was measured to be a balanced amount for both the unknown and the reagents, it is possible that the solution was not mixed thorough enough to receive a proper reaction. Waiting an appropriate amount of time for the right reaction to occur may also have a cause for error in the