L. B Agar Lab Report

L.B. Agar Lysogeny broth (LB), a nutritionally rich medium, is primarily used for the growth of bacteria. The abbreviation is commonly taken to mean Luria broth, Lennox broth, or Luria-Bertani medium. According to its creator Giuseppe Bertani, the abbreviation LB was actually intended to stand for lysogeny broth (1). The formula of the LB medium was published in 1951 and continues to be the most common media used today (2). There are several common formulas of LB. Although they are different, they generally share a similar list of ingredients used to promote growth in bacteria, including the following: Peptides and casein peptones Vitamins (including B vitamins) Trace elements (e.g. nitrogen, sulfur, magnesium) Minerals
Peptides and peptones are provided by tryptone. Tryptone is used to provide essential amino acids to the growing bacteria. Vitamins and certain trace elements are provided by yeast …show more content…
Recent recipes typically leave out the glucose.

Streak of LB Agar Plate with Unknown T1
OBJECTIVE: Streak a LB Agar plate with unknown T1.
METHOD: 1) Used aseptic technique to streak a plate with the unknown bacteria T1. 2) The loop was dry sterilized and cooled. 3) A small loopful of bacteria was taken. 4) The loop was gently rubbed on the first half of the plate in a tight zig-zag fashion. 5) The loop was dry sterilized and cooled again. From the first half of the plate, the loop was dragged through to the second region of the plate in a tight zig-zag fashion. 6) The loop was dry sterilized and cooled again. The loop was pulled through the second region to the third region and streaked in a loose zig-zag fashion. 7) The plate was incubated overnight at 37 degrees Celsius.
RESULTS: Independent colonies were formed.
CONCLUSION: T1 was is not a fast growing