Kanamycin Sulphate And Rifampicin Synthesis Essay

2078 Words 9 Pages
Kanamycin sulphate and rifampicin were purchased from Hangzhou Dayangchem Co., Ltd., Zhejiang, China. Acetonitrile and ethanol (High performance liquid chromatography, HPLC, grade) were purchased from Merck, Germany. Sodium dihydrogen phosphate, phenylisocyanate, triethylamine, orthophosphoric acid (analytical reagent grade) and silicone oil (viscosity 10 cSt) were purchased from Sigma–Aldrich, St. Louis, USA. Size 3 hard gelatin capsules were kindly donated by Capsugel Co., Ltd., Tokyo, Japan. Fresh Milli-Q water was collected and filtered through 0.45 μm membrane filter before use. Preparation of powders by spray-drying
Powder particles were produced using a Buchi B-290 Mini Spray-Dryer (Buchi Labortechnik AG, Flawil, Switzerland) with a high performance cyclone in a closed-mode. Feed solutions of kanamycin (as sulphate) or/and rifampicin were prepared in a co-solvent system of ethanol and water (70:30, v/v). Rifampicin was first dissolved in ethanol; kanamycin was dissolved in water. The two solutions were then mixed (at rifampicin and kanamycin ratio of 40 and 60% w/w) and sonicated for 5
…show more content…
In both cases the injection volume of the sample was 20 µL with the respective run time of 20 and 10 min for kanamycin and rifampicin. Kanamycin samples (500 µL) in water were derivatized by mixing with 250 µL of freshly prepared phenyl isocyanate solution (0.5% in acetonitrile) and 250 µL of freshly prepared triethylamine (0.5% in acetonitrile) and heating the solution at 70 °C for 10 min on a shaking water-bath (Grant Instruments Ltd., Barrington, Cambridge, CB25OZ, England). Rifampicin samples were directly prepared in methanol. The wavelengths for detection were 240 and 238 nm, respectively for kanamycin and

Related Documents