Joinville is a city located in Southern Brazil with 516,288 inhabitants, according to the last national census. The Joinville Stroke Registry is an ongoing population-based registry databank started in 2005 and supported by law since 2013. The registry uses the methodology proposed by Sudlow and Warlow as well as the Stroke-Steps modular program proposed by the WHO (first step for all hospital cases, second step for checking of death certificates and third step to ascertain mild events). The detailed methods of cohort recruitment and data collection procedures have been previously described (7), the Joinville Stroke Registry is associated to Joinville Stroke Biobank, which storage DNA samples from all patients of Joinville Stroke Registry. The baseline characteristics for all patients and controls included in study are shown in Table 1.
Ethical considerations
The study was approved by the Institutional Ethical Review Board under protocol number 1.396.172.
Inclusion Criteria
Cases include, ischemic strokes (IS) subtypes LAAS and CE classified according to the modified Trial of Org 10172 in Acute Stroke Treatment (TOAST) …show more content…
Thereafter, genomic DNA was obtained by the classical “phenol-chloroform” procedure for total DNA extraction. DNA was resuspended in 200 µL of buffer TE (10 mMTris-HCl; 1 mM EDTA; pH 8.0). The purity and yield of DNA extracted were evaluated by spectrophotometry at 260 and 280 nm (Epoch, Biotek Instruments, Winooski, USA). A260/A280 ratios in the range 1.8 – 2.0 were considered for long-term storage. DNA was diluted to a concentration of 10ng/mL before we genotyped the samples. Genotypes were determined by Real Time Polymerase Chain Reaction (qPCR) with employment of TaqMan® probes for the two genetics variants of the study. The reaction was performed using procedures recommended by
Ethical considerations
The study was approved by the Institutional Ethical Review Board under protocol number 1.396.172.
Inclusion Criteria
Cases include, ischemic strokes (IS) subtypes LAAS and CE classified according to the modified Trial of Org 10172 in Acute Stroke Treatment (TOAST) …show more content…
Thereafter, genomic DNA was obtained by the classical “phenol-chloroform” procedure for total DNA extraction. DNA was resuspended in 200 µL of buffer TE (10 mMTris-HCl; 1 mM EDTA; pH 8.0). The purity and yield of DNA extracted were evaluated by spectrophotometry at 260 and 280 nm (Epoch, Biotek Instruments, Winooski, USA). A260/A280 ratios in the range 1.8 – 2.0 were considered for long-term storage. DNA was diluted to a concentration of 10ng/mL before we genotyped the samples. Genotypes were determined by Real Time Polymerase Chain Reaction (qPCR) with employment of TaqMan® probes for the two genetics variants of the study. The reaction was performed using procedures recommended by