Java Plum Berry Case Study

REVIEW OF RELATED LITERATURE of Eugenia jambolana Lam.,
Java plum berry begin to exist in India where it is originally called as Jamun . Eugenia jambolana Lam., is the scientific name of this plant. Jambolan , Jambas , Jambul , Jamali , Jambul, Black Plum, Java Plum, Indian Blackberry, Jamblang, etc. are just some of the other common names of Java plum berry from different parts of the world . It belongs to the Myrtaceae family or Syzygium cumini (also called as Syzygium jambolanum and Eugenia cumini). Duhat is the Filipino term for Java plum berry . It usually bears fruits between the month of June and July( Tyagi,2012).
Duhat tree is located everywhere especially in tropical regions like Southeast Asia and Eastern Africa . It
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There are different uses of stains. One of these is by defining and examining: bulk tissues through highlighting muscle fibers or connective tissue, and cell populations by classifying different blood cells or organelles within the cells. (Staining. (2017, February 28). In Wikipedia, The Free Encyclopedia. Retrieved 11:28, April 11, 2017, from
Cell staining is used to better visualize cells and its components such as nucleus and cell wall under a microscope which would otherwise not be seen clearly. Stains can be used both on living and non-living or fixed cells. Enumeration of cells to determine biomass is done through cell staining (Bruckner,
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Genuine work on histological dye staining, though, started not before the second half of 19th century in which carmine or cochineal was the most used dye. General stains will dye the tissue uniformly while selective stains will dye only the tissue components in which they have natural affinity for. Dyes can be roughly divided into acidic dyes and basic dyes. Basic dyes stain acidic components while acidic dyes stain basic components. P. Ehrlich used a method for mixing acidic and basic dyes that has become an important milestone in the development of staining techniques. The intensity and contrast of microscopic pictures are determined by the quality of the dye solution and the procedure used. There are two viewpoints on the division of dyes – chemical and practical classification. In chemical classification, organic dyes are divided into azo dyes, nitro and nitroso dyes, chinone dyes, di- and triphenylmethan dyes, xanthenes dyes, acidrine dyes, azine dyes, oxazine dyes, thiazine dyes, and vat dye. On the other hand, practical classification divides dyes based on their coloring effect: basic dyes, acidic dyes, substantive dyes, and mordant dyes (Kuhlmann,

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