2.13.3. Isolation and identification of IBDV:
Isolation of IBDV in chicken embryos:
For initial isolation of IBDV the CAM was more sensitive route than the yolk and allantoic sac routes. Gross lesions observed in embryo were edematous distention of the abdominal region, cutaneous congestion and petechial hemorrhages, particularly along feather tracts, occasional hemorrhages on toe joints and in cerebral region, mottled-necrosis and ecchymotic hemorrhages in the liver (latter stages) pales (para-boiled) appearance of the heart, congestion and some mottled necrosis of kidneys, extreme congestion of lungs, and pale spleen. The CAM had no plaques, but small hemorrhagic areas are present (Hitchner, 1970). Embryos inoculated with IBDV usually died from the third to fifth day on wards after inoculation, with dwarfing, edema, congestion and hemorrhages on the subscutis and the region of the kidneys, also swelling of the liver with greenish discoloration and necrosis, enlargement of the spleen and small white opaque pocks on the CAM (Faragher, 1972).
Lesions induced in embryos by IBDV strains differed from those induced by standard isolated. Spleenomegally and …show more content…
Many IBDV isolates have been adapted to replicate and produce CPE in primary cell cultures of chicken embryo origin, including chicken embryo kidney cells (CEK) and chicken embryo fibroblasts (CEFs) (McNulty et al., 1979).
IBDV peak titers were obtained 48 hours post-inoculation in CEF cell cultures with cytopathic effect and plaque formation after serial passages in embryonated eggs and then in CEF cultures (Cho et al., 1979). Turkey and duck embryo cells, mammalian cell line derived from rabbit kidneys (RK-13), monkey kidneys (Vero) and baby grivet monkey kidney cells (BGM-70) were susceptible for virus growth besides chicken cells (Lukert and Saif,
Isolation of IBDV in chicken embryos:
For initial isolation of IBDV the CAM was more sensitive route than the yolk and allantoic sac routes. Gross lesions observed in embryo were edematous distention of the abdominal region, cutaneous congestion and petechial hemorrhages, particularly along feather tracts, occasional hemorrhages on toe joints and in cerebral region, mottled-necrosis and ecchymotic hemorrhages in the liver (latter stages) pales (para-boiled) appearance of the heart, congestion and some mottled necrosis of kidneys, extreme congestion of lungs, and pale spleen. The CAM had no plaques, but small hemorrhagic areas are present (Hitchner, 1970). Embryos inoculated with IBDV usually died from the third to fifth day on wards after inoculation, with dwarfing, edema, congestion and hemorrhages on the subscutis and the region of the kidneys, also swelling of the liver with greenish discoloration and necrosis, enlargement of the spleen and small white opaque pocks on the CAM (Faragher, 1972).
Lesions induced in embryos by IBDV strains differed from those induced by standard isolated. Spleenomegally and …show more content…
Many IBDV isolates have been adapted to replicate and produce CPE in primary cell cultures of chicken embryo origin, including chicken embryo kidney cells (CEK) and chicken embryo fibroblasts (CEFs) (McNulty et al., 1979).
IBDV peak titers were obtained 48 hours post-inoculation in CEF cell cultures with cytopathic effect and plaque formation after serial passages in embryonated eggs and then in CEF cultures (Cho et al., 1979). Turkey and duck embryo cells, mammalian cell line derived from rabbit kidneys (RK-13), monkey kidneys (Vero) and baby grivet monkey kidney cells (BGM-70) were susceptible for virus growth besides chicken cells (Lukert and Saif,