Diabetic Lab Report

Abstract Type 2 Diabetes is a disease that is normally characterized by how the body has a lack of sensitivity to insulin and how the cells bind to the insulin, this causes a person’s response to glucose to change. The glucose levels in diabetics are much higher than the average person due to the lack of sensitivity that they have to insulin. In this experiment, we tested the hypothesis that people who are diabetic will have a higher blood glucose concentration and light absorbance than non-diabetics as tested by a spectrometer test. The absorbency rate was determined by a spectrometer test which was set to a wave length of 504nm. Blood samples were taken over time periods of 0,30,60,90 and 120 minutes. The rate of glucose in the diabetic …show more content…
The wavelength of 504nm is the length at which red dye is best absorbed. The red dye was used as our model for the blood glucose samples. With the chamber being emptied and closed, the left knob was adjusted until the meter read infinity absorbance. A blank cuvette was then cleaned by using a kimwipe and placed inside the sample chamber with the white dot facing forwards. The right knob was adjusted until it read zero absorbance. We blanked the spectrometer because we wanted to read only the blood glucose samples absorbance. The water’s absorbance was subtracted so we would only be reading the absorbance of the red dye. The first numbered cuvette was then cleaned with a kimwipe and inserted into the spectrometer and the chamber was closed on top of it. The absorbance level was then read from the meter, with the needle being aligned with the cuvettes reflection. The value was then recorded. This step of inserting the cuvettes was then repeated for the rest of the samples. After read each absorption the data was represented in a Microsoft excel graph to see the correlation. The cuvettes were rinsed out and returned to the rack on the lab stations (Weinstein,