Results:
At the start of the experiment, the initial flow rate was established as 20 ml min-1. This means that there is a steady flow of fluid or blood in your body (in this case, water) at a rate of 20ml min-1. In order to find the final concentration of the Eosin (2,5 mg ml-1) in the “blood” after 60 minutes a calibration curve had to be created by making up seven different solutions of eosin (11,25 μg ml-1) and water to compare it against their absorbance that was calculated in a spectrophotometer. Figure 1 shows this correlation. It is clearly evident that the higher the concentration of eosin in a test tube, the higher the absorbance of that test tube will be. It shows that absorbance is directly proportional to concentration. This …show more content…
The number of half-lives in 45 minutes was calculated as 2,42 therefore resulting in a 18,7% left after 45 minutes, therefore, after using the equation, 81% was calculated. This is the percentage of the “drug” eliminated from the system after 45 minutes.
Discussion:
The experiment has been completed and the results were recorded as accurately as possible. The results we obtained seemed to be in favor of the hypothesis we had set out initially. It seems to show that as time increased, the concentration of the “drug” left in the blood system decreased. It also shows that, the higher the concentration at a specific time interval, the higher the absorbance value will be. A reason for this is because light absorbance is related to color, as well as, concentration, in that, when the light passes through the sample of material, (in this case a mixture of eosin and water), the photons of light hit molecules of the mixture and it then dissipates, thus increasing the absorbance. The wavelength used for this …show more content…
The history of staining. Chapter 2 in: Horobin RW, Kiernan JA (eds.), Conn’s Biological Stains. 10th ed. Oxford, BIOS Scientific Publishers Ltd: 2002,15-22. Glomerular filtration. Mosby 's Medical Dictionary, 8th edition. (2009). Retrieved October 27 2015 from http://medical-dictionary.thefreedictionary.com/glomerular+filtration "Absorption" 01 July 2009. HowStuffWorks.com. 27 October 2015. Bartholomew, EF, Martinin, FH, Nath, JL (2012). Fundementals of Anatomy & Physioloy, 10th edition, global edition. Pearson, San Francisco, United States of America. (p.661, 999-1037) Gibaldi, M. Biopharmaceutics and Clinical Pharmacokinetics. 3rd Edition. Lea & Febiger, Philadelphia, 1984,
At the start of the experiment, the initial flow rate was established as 20 ml min-1. This means that there is a steady flow of fluid or blood in your body (in this case, water) at a rate of 20ml min-1. In order to find the final concentration of the Eosin (2,5 mg ml-1) in the “blood” after 60 minutes a calibration curve had to be created by making up seven different solutions of eosin (11,25 μg ml-1) and water to compare it against their absorbance that was calculated in a spectrophotometer. Figure 1 shows this correlation. It is clearly evident that the higher the concentration of eosin in a test tube, the higher the absorbance of that test tube will be. It shows that absorbance is directly proportional to concentration. This …show more content…
The number of half-lives in 45 minutes was calculated as 2,42 therefore resulting in a 18,7% left after 45 minutes, therefore, after using the equation, 81% was calculated. This is the percentage of the “drug” eliminated from the system after 45 minutes.
Discussion:
The experiment has been completed and the results were recorded as accurately as possible. The results we obtained seemed to be in favor of the hypothesis we had set out initially. It seems to show that as time increased, the concentration of the “drug” left in the blood system decreased. It also shows that, the higher the concentration at a specific time interval, the higher the absorbance value will be. A reason for this is because light absorbance is related to color, as well as, concentration, in that, when the light passes through the sample of material, (in this case a mixture of eosin and water), the photons of light hit molecules of the mixture and it then dissipates, thus increasing the absorbance. The wavelength used for this …show more content…
The history of staining. Chapter 2 in: Horobin RW, Kiernan JA (eds.), Conn’s Biological Stains. 10th ed. Oxford, BIOS Scientific Publishers Ltd: 2002,15-22. Glomerular filtration. Mosby 's Medical Dictionary, 8th edition. (2009). Retrieved October 27 2015 from http://medical-dictionary.thefreedictionary.com/glomerular+filtration "Absorption" 01 July 2009. HowStuffWorks.com. 27 October 2015. Bartholomew, EF, Martinin, FH, Nath, JL (2012). Fundementals of Anatomy & Physioloy, 10th edition, global edition. Pearson, San Francisco, United States of America. (p.661, 999-1037) Gibaldi, M. Biopharmaceutics and Clinical Pharmacokinetics. 3rd Edition. Lea & Febiger, Philadelphia, 1984,