Essay about Instrumnt Profile

1831 Words Dec 2nd, 2014 8 Pages
Varian Cary Eclipse Spectrofluorometer: (Fluorescence, Phosphorescence, Chemiluminescence)

Table of Contents:

Chapter 1: Introduction…………………………………………………….3 a. General Characteristics…………………………………………...3 b. Signal Theory…………………………………………………….3/4 c. Sample Type……………………………………………………...5 d. Advantages……………………………………………………….5 e. Disadvantages/Limitations……………………………………….5

Chapter 2: Instrumentation………………………………………………....6 a. Block Diagram…………………………………………………...6/7 b. Internal Design…………………………………………………..6/7 c. External Design…………………………………………………6/7

Chapter 3: Operating Instructions………………………………………….8 a. Start Up/Shut Down…………………………………………......8 b. Precautions…………………………………………………….....8 c.
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Advantages
The Varian Cary Eclipse Spectrofluorometer can be set to run on three different settings including chemiluminescence, fluorescence, and phosphorescence. Instead of scanning the wavelengths the wavelengths can be optimized to determine the optimized intensity. Since Simple Reads will help with the optimizing protocol the running the samples requires minimal preparation except for preparing the samples and standards. e. Limitations
Both internal and external factors can affect fluorescence measurements. Internal factors include those within the sample matrix, such as the structure and rigidity of the molecule, functional groups, solution pH, temperature, concentration, and internal scattering. External factors include instrument and method sensitivity.
Temperature variation has been known to affect the wavelength at which a molecule fluoresces and the width of the fluorescence spectra. Changes in temperature affect the viscosity of the medium and hence influence the number of collisions between fluorescing molecules and solvent molecules.

Chapter 2: Instrumentation
General Spectrofluorometer Design
Xe Flash Lamp
Xe Flash Lamp

Chapter 3: Operating

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