Inhibition of cash crops and other plants immune system response has been linked to a bacterial infection. Researchers in the Department of Biology, Howard Hughes Medical Institute, Curriculum in Genetics and Molecular Biology, Department of Microbiology and Immunology, and the Carolina Centre for Genome Sciences at the University of North Carolina, and the Department of Biological Chemistry at John Innes Centre, have uncovered a correlation between bacterial protein, a molecule that performs most functions within cells (the general units of life).
Erica J. Washington and her team used methods such as the use of microscopes, separation of proteins based on size, and production of the 3D experimental protein by utilizing closely related proteins as models. Using these techniques, they could determine where the protein in question performed its function, determine amount of ethylene production as well as the impact of the protein on other factors, and define …show more content…
These two proteins are important to ethylene production as they are used in part of the Yang cycle which is a process that reproduces one of the essential components of ethylene production. MTN1 and MTN2 are known to exist in the cell’s membrane and to establish an interaction between HopAF1 and MTN1/MTN2, a type of microscope was used that allowed for HopAF1 to be dyed and followed. From this microscopic viewing, they concluded that HopAF1 interacts with MTN1/MTN2 on the cell membrane. Due to the interaction, they also concluded that HopAF1 inhibits ethylene production. This is evident in figure 3A (Washington et al., 2016) where ethylene production (in pL/mg fresh weight) was graphed against a kind of bacteria lacking the transport system required from HopAF1. In order get HopAF1 in the cell, a ‘delivery vehicle’ was added into the cell that was capable of
Erica J. Washington and her team used methods such as the use of microscopes, separation of proteins based on size, and production of the 3D experimental protein by utilizing closely related proteins as models. Using these techniques, they could determine where the protein in question performed its function, determine amount of ethylene production as well as the impact of the protein on other factors, and define …show more content…
These two proteins are important to ethylene production as they are used in part of the Yang cycle which is a process that reproduces one of the essential components of ethylene production. MTN1 and MTN2 are known to exist in the cell’s membrane and to establish an interaction between HopAF1 and MTN1/MTN2, a type of microscope was used that allowed for HopAF1 to be dyed and followed. From this microscopic viewing, they concluded that HopAF1 interacts with MTN1/MTN2 on the cell membrane. Due to the interaction, they also concluded that HopAF1 inhibits ethylene production. This is evident in figure 3A (Washington et al., 2016) where ethylene production (in pL/mg fresh weight) was graphed against a kind of bacteria lacking the transport system required from HopAF1. In order get HopAF1 in the cell, a ‘delivery vehicle’ was added into the cell that was capable of