Importance Of Histopathology

991 Words 4 Pages
Gissela Ruiz
Histopathology terms
Dr. Agapito

Reference:
E. Gupta, P. Bhalla, N. Khurana, T Singh, “ Histopathology for the diagnosis of infectious diseases” Indian Journal of Medical Microbiology. (2009) 27(2):100-6.
Questions:
1. What is Histopathology?
Histopathology is the examination of a tissue biopsy to try and find the cause of the infected organism.
2. What is the importance of a Histopathological Examination?
The Importance of a Histopathological examination is to try and find a faster way to examine infections and diseases. So it can help figurize the disease/ infection you have.
3. What are the setbacks of Microbiologist trying to identify organisms?
The setbacks of Microbiologist trying to identify organisms are trying to identify
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10. What are the common stains used to identify a fungal infection?
The common stains used to identify a fungal infection is tissue, muscle, bone marrow, lung, and lymph node.

11. Please compare and contrast Gram positive versus Gram negative bacteria? ( use additional resources)
The difference and similarities of a gram positive versus Gram negative bacteria is that the gram negative is really difficult to see because of the lack of contrast between the bacteria and the counterstain. Both the Gram negative and positive are different, but they have that one is superior then the other.

12. What is the ideal staining for identifying a Gram positive bacteria – Please explain your answer?
The ideal staining for identifying a Gram positive bacteria is brown-brenn gram stain is preferred to detect the positive bacteria.

13. What is the ideal staining for identifying a Gram negative bacteria – Please explain your answer?
The ideal staining for identifying a Gram negative bacteria is the brown-brenn modification is superior for the detection of the gram negative
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Explain in detail, In situ Polymerase Chain reaction (PCR) technique?
In situ Polymerase Chain reaction is a method that may be used to amplify the nucleic acid, so it can use PCR in the intact tissue to detect and localize the amplified product.

17. Identify the advantages and disadvantages of ImmunoHistochemistry, In situ Hybridization, and In situ Polymerase Chain reaction?
The advantages of ImmunoHistochemistry in situ hybridization, and situ Polymerase Chain reaction is how they are all able to to detect what they need like ImmunoHistochemistry detects stains, In situ Hybridization detects DNA chromosomes and tissue, and situ Polymerase Chain detects amplified products. The disadvantages of ImmunoHistochemistry in situ hybridization, and situ Polymerase Chain reaction is that they all have there limitations in specificity exist.

18. What is the conclusion of the author?
The conclusion of the author is that the unity of histopathology in diagnosing infectious disease have it all establish, and how the diagnostic histopathology is being developed more rapidly and specific. And if microbiologist, pathologists and clinicians work together they can easily diagnose difficult

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