Importance Of Gram Staining In Microbiology

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Introduction:
Gram staining is an essential staining technique used in microbiology. This type of staining falls under the differential staining category, as it distinguishes between two types of bacteria. Gram staining originated from Christian Gram (1). The discovery of this technique originated from Gram’s examination of lung tissue from patients who died of pneumonia. He was fascinated by differences between the ability of types of bacteria’s to uptake stain (2). Yet, the technique that he created was not the finalized version of the gram staining technique that is used today. Later on, Carl Weigert added the final step of the gram staining process. Weigert incorporated the step involving the counterstaining of bacteria with Safranin (3). Currently, the gram staining technique is used to distinguish the characteristics of bacteria.
In this experiment, gram staining was used to compare the composition of the
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Even when both types of bacteria were mixed together, gram-negative and gram-positive, the distinct characteristics of all of the cells were shown. Thus, supporting the importance of proper gram-staining techniques. Gram-staining is essential in identifying and differentiating bacterial species. In the case of the collected unknown, gram-staining was critical in identifying the bacteria as possibly being Staphylococcus aureus. This type of bacteria is spherically shaped and grows in grape-like clusters. It is also a gram-positive bacteria. The characteristics of S. aureus were displayed within the slide of the unknown colony. Moreover, S. aureus is found within the nose and respiratory tract (8). Thus suggesting that the bacteria was released from the nasal passage onto the agar plate as the cough occurred. Therefore, one can conclude that S. aureus was the bacteria collected from the cough within the agar plate

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