Identification, Analyitical Data Collection of Natural Products

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Natural Products Experiment
Niki Robinson 10008562

Practical 2: Identification and quantification of taxifolin from milk thistle

Aim: to Identify and quantify the amount of taxifolin from three samples Silymarin stock solution, taxifolin and silybum marianum extract. `

Methodology: Using auto-pipette (20-100 microlitre), pipetted 0.06 mg of Taxifolin was added to 10ml conical flask and made up to the mark (10ml) with H2O.This procedure was repeated with the same amounts foe silymarin stock solution and silybum marianum extract. Taxifolin was transferred to 2ml brown bottle for HPLC auto sampler, this was also done to silymarin stock solution. Silybum marianum extract was filtered via a syringe filter (0.2 microlitre).The three
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HPLC Conditons when taxifolin, silymarin and silybum methanol extract run
C18 column (4.6x 150mm) Autosampler
Wavelength: 280nm
Solvent was 0.5% phosphoric acid and methanol.
The sample weight /volume: 1000/1000 Injected volume 10 ┬Ál Elution time: 32 min
Taxifolin 0.06 Dilution HPLC Chromatogram
Figure 3 Taxifolin HPLC
Methanol Extract 0.06 Dilution HPLC Chromatogram
Figure 4 Methanol Extract HPLC

Silymarin Stock Solution 0.06 Dilution HPLC Chromatogram

Figure 5 Silymarin Stock Solution HPLC

Table 2 Calculations of amount obtained from samples Taxifolin Standard samples given on table 1 and figure 1 | Concentration (mg/ml) | y = Peak Area (mUA) | y272.59=x (Taxifolin)5 s.f | 0 | 0 | 0 | 0 | 0.0036 | 1.876 | 0.00688213 | 0.0070 | 0.0072 | 2.862 | 0.01049929 | 0.1050 | 0.0144 | 4.518 | 0.01657434 | 0.1660 | 0.0288 | 8.074 | 0.29619575 | 0.2962 | 0.0576 | 15.273 | 0.05602920 | 0.0560 | Unknown concentration Samples shown in figure 3-5 | Dilution/ Group | | | | y = Peak Area (mUA) | y272.59=x 5 s.f | 0.06 (Group 12) | | | Taxifolin | 1.390 | 0.00599233 | 0.0060 | Silymarin | 0.122 | 0.00044756 | 0.0004 | Methanol Extract | 0.438 | 0.00160808 | 0.0020 | | | | 0.06 (Group 1) | | | Taxifolin | 1.631 | 0.00598335 | 0.0060 | Silymarin | 0.044 | 0.00016142 | 0.0002 | Methanol Extract | 0.032 | 0.00011739 | 0.0001 | | | | 0.06 (group 8) | | | Taxifolin | 2.225

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