1.)During the lab and based on recorded observations it can be deduced that the enzyme catalase found in potatoes only reacted to hydrogen peroxide. This is do to the fact that before 10 ml of hydrogen peroxide was added to each test tube residing 3 potato samples and the designated liquid, no reaction was observed. Thus demonstrating the catalase's inability to react to vinegar, soda, water, detergent, and Na OH. Furthermore, shortly after the hydrogen peroxide was added to the liquids of diverse ph levels, a rapid and prominent throng of bubbles began to appear, which demonstrates the mediate reaction the hydrogen peroxide had on catalase.…
The Effects of an Enzyme, Catechol Oxidase, on the Conversion of Four Different Substrates to Pr oduce Benzoquinone. Amir Akhras Lab partners: Monica Elabed, Andres Velazquez, and Roland Rencz Biology 201 Instructor: Dr. Kara Nuss October 28, 2014 ABSTRACT Enzymes are catalysts that speed the reaction of substrates to produce products. They achieve this by lowering the energy of activation (EA) but they do not change the nature of the reaction. The objective of this experiment was to test which of the four substrates will produce benzoquinone based on their molecular formula and absorb the most light.…
Nine tests tubes were used for the effects of temperature and effects of pH on the enzyme activity. Mixing two tubes at a time at four different temperatures and recording the absorbance given in twenty-second intervals found the effects that temperature and pH has on peroxidase. Tubes 2 and 3 were tested at 4°C, tubes 4 and 5 were tested at 23°C, tubes 6 and 7 were tested at 32°C and tubes 8 and 9…
The effect of altering temperature on the rate of liver enzyme activity is measured in this experiment to be able to visualize how temperature in the environment and in the body can manipulate the rate of enzyme activity. Enzymes are protein catalysts that lower the amount of activation energy required to start a biochemical reaction. According to Dr. Meyertholen (2015), enzymes are structured to work with certain substrates, meaning that the structure determines the function of the enzyme. If the enzymes shape is altered, they become denatured. Denaturation of an enzyme can occur with changes in factors such as temperature, pH, or salinity.…
Liver Lab By: Jonah Stillman Background Information: Catalase enzymes are inside of nearly every living organism that is exposed to oxygen. Some examples of catalase enzymes are bacteria, plants, and animals. The main job of a catalase enzyme is to catalyze the decomposition of hydrogen peroxide to water and oxygen. When an enzyme is exposed to hydrogen peroxide or certain other chemicals it is prone to react. Catalase enzyme reactions also depend on the temperature they are exposed to.…
The Effects of Competitive Inhibitors on Catechol Oxidase Enzyme Activity. Jaley Willingham, 2015, Functional Biology, Texas State University, San Marcos, 78666. Abstract Competitive inhibitors may have an effect on enzyme activity.…
For the first part of our lab part A we were focusing on observing a normal catalase reaction. We used a normal piece of liver to start it off the rate of reaction for the liver was about a three on a scale from 0-5, 0 being nothing and 5 being fast.…
The focus of this lab was to identify an unknown organism based on its characteristics and the results from each of the tests. There will be various of test to choose from in order to identify the unknown organism, which will eliminate numerous possibilities and narrow it down to one. All the fundamental skills that we have learned and practiced in the lab will be used to perform on our unknown such as aseptic technique, microscopic examination, the use of differential media, and determining if it’s positive or negative. Performing aseptic techniques is the most crucial step that requires the utilizing of transferring, inoculating, and storing bacterial cultures and media. Aseptic technique is defined as procedures that prevent contamination…
Discussion Enzymes are biological catalysts that help speed chemical reactions. In part A (table 2), it was seen that a normal reaction of catalase and hydrogen peroxide resulted in the release of bubbles (oxygen gas) and the release of energy in the form of heat which shows that the reaction was a exothermic reaction. The reaction was given a reaction rate of four and acted as the control with which we could compare the other results to. When testing whether or not catalase is reusable in part B (table 3), the results show that it is reusable as it reacted more than once. This matches our hypothesis as the enzyme should remain unchanged after the reaction and be able to react again afterwards.…
In this part of the lab, we pour 20 mL of Hydrogen Peroxide and 1 mL of yeast catalase in the reaction chamber and then submerge it in the water bath. After it was done, it can be concluded that the gas levels increased because when we first started timing it, the gas levels were at 76, but as time went by, the gas levels started to increase and in the end of the last interval, the gas level increased all the way up to 100. This result occurred because there were many active sites that were empty for the substrate to bind to and since they binded to the active sites, it started increasing a lot at the beginning. However, when the active sites started getting more occupied, the substrate could not bind to as much active sites, so the gas levels started to increase alittle less as time went on and in the end, there was no active sites that were…
Two centimeters-cubed of the source of the catalase enzyme, yeast, was added into the 100 milliliter graduated cylinder 3. Two milliliters of the hydrogen peroxide were put in a boiling tube 4. The graduated cylinder and the boiling tube were both placed into a water bath that had a temperature of 82 degrees Fahrenheit 5. Once they were at the same temperature, I poured the hydrogen peroxide into the graduated cylinder. At the same time, I noted the overall initial volume of the of fluid in the graduated cylinder 6.…
Specific environmental conditions may play a role in how enzyme activity is affected. We conducted an experiment to observe whether the change in temperature altered the reaction rate of an enzyme. The activity rate of our enzyme, catechol oxidase, was collected by analyzing the changes in absorption rate of the enzyme in a buffered catechol solution after being placed in four different temperatures. In which one solution remained out in room temperature being to be our negative control. Each of the four solutions were placed in their respective temperature environment and for every two minute intervals, over a twenty minute time process, we read the concentration levels through a spectrophotometer.…
The experiment conducted was to observe the enzyme catalysis of Horseradish Peroxidase with different determining factors. In this experiment the horseradish peroxidase enzyme should have an optimal temperature of 35°C, should have an optimal pH of 6, and should catalyze rapidly with the substrate and enzyme concentration. We were able to observe that the enzyme reacts best with its optimal temperature of 35° C and optimal pH of 6. Moreover, when there’s a high the substrate concentration and a high enzyme concentration we can observe a rapid enzyme catalysis. The hypothesis was partially falsified for the optimal pH and the optimal temperature were both different than that stated in the hypothesis.…
The purpose of this experiment is to conduct and test the effects of catechol oxidase with varying temperatures. Based on many scientific journals, changes on different enzymes are affected by temperature (Pao-Huei et al. 2016). These concluded that the reaction of catechol oxidase is accelerated…
Analysis Questions: 1. The primary reaction catalyzed by catalase is the decomposition of hydrogen peroxide to form water and oxygen, which occurs spontaneously, but not at a very rapid rate. Write a balanced equation for this reaction. Label the reactant and the product. (Remember that catalase is not a reactant or a product and can be written over the arrow separating the reactant from the products.)…