Materials and Methods
Chemicals and kits
Chemicals: CCL4 dissolved in corn oil at 1: 1 percent was injected intraperitonealy at a dose of 1.2 ml/ kg of body weight 3 times a week.
Kites:
Diagnostic kits for serum total proteins, albumin, total lipid, triglyceride, total cholesterol, HDL-c, LDL-c, VLDL-c, alanine aminotransferase (ALT) and aspartate amino transferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN), uric acid and creatinine were purchased from ELIPSE, United diagnostic industry, UDI, Dammam, Saudi Arabia. Paraffin oil, carbon tetrachloride (SpectrosolW BHD chemicals ltd pool, England) and other chemicals and solvents were of highest grade commercially available.
Camel’s milk
Camel’s milk samples were collected daily …show more content…
Experimental groups and protocol
The rats were divided randomly into 4 groups comprising 6 rats in each group and fed the same diet throughout the experimental period. The experimental design is described as fellow:
Group I: Rats fed only with basal diet and tap water and injected i/p with Paraffin oil, this group served as control 1.
Group II: Rats fed normal basal diet, injected i/p with Paraffin oil and received camel’s milk (100 ml/24 h/cage) as their sole source of drinking water, this group served as control 2.
Group III: Rats fed basal diet and tape water and intoxicated with CCl4 (1 ml/kg b.wt.), 1:1 diluted with paraffin oil on first two days of the experiment.
Group IV: Rats fed basal diet and intoxicated with CCl4 (1 ml/kg b.wt.), 1:1 diluted with paraffin oil on first two days of the experiment and then treated with camel’s milk (100 ml/24 h/cage) as their sole source of drinking water.
Blood and tissue …show more content…
Elevation of bilirubin levels may be due to interfering of CCL4 with bilirubin synthesis and discharge of bile acids , cholesterol and bilirubin (38). CCL4 may affects the integrity of liver cells , by the same manner it effect on the structure and function of erythrocyte membrane and increase the erythrocyte fragility , this in turn leads to erythrocyte hemolysis and hemoglobin breakdown (39).On other hand, increase of bilirubin in CCL4 treated rats may be attributed to hepatic damage which ends up in cirrhosis associated to oxidative stress where oxidation of fatty acids in hepatocytes membrane and the formation of a thick extracellular matrix are the main causes of the loss of the homeostatic equilibrium in the liver
Chemicals and kits
Chemicals: CCL4 dissolved in corn oil at 1: 1 percent was injected intraperitonealy at a dose of 1.2 ml/ kg of body weight 3 times a week.
Kites:
Diagnostic kits for serum total proteins, albumin, total lipid, triglyceride, total cholesterol, HDL-c, LDL-c, VLDL-c, alanine aminotransferase (ALT) and aspartate amino transferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN), uric acid and creatinine were purchased from ELIPSE, United diagnostic industry, UDI, Dammam, Saudi Arabia. Paraffin oil, carbon tetrachloride (SpectrosolW BHD chemicals ltd pool, England) and other chemicals and solvents were of highest grade commercially available.
Camel’s milk
Camel’s milk samples were collected daily …show more content…
Experimental groups and protocol
The rats were divided randomly into 4 groups comprising 6 rats in each group and fed the same diet throughout the experimental period. The experimental design is described as fellow:
Group I: Rats fed only with basal diet and tap water and injected i/p with Paraffin oil, this group served as control 1.
Group II: Rats fed normal basal diet, injected i/p with Paraffin oil and received camel’s milk (100 ml/24 h/cage) as their sole source of drinking water, this group served as control 2.
Group III: Rats fed basal diet and tape water and intoxicated with CCl4 (1 ml/kg b.wt.), 1:1 diluted with paraffin oil on first two days of the experiment.
Group IV: Rats fed basal diet and intoxicated with CCl4 (1 ml/kg b.wt.), 1:1 diluted with paraffin oil on first two days of the experiment and then treated with camel’s milk (100 ml/24 h/cage) as their sole source of drinking water.
Blood and tissue …show more content…
Elevation of bilirubin levels may be due to interfering of CCL4 with bilirubin synthesis and discharge of bile acids , cholesterol and bilirubin (38). CCL4 may affects the integrity of liver cells , by the same manner it effect on the structure and function of erythrocyte membrane and increase the erythrocyte fragility , this in turn leads to erythrocyte hemolysis and hemoglobin breakdown (39).On other hand, increase of bilirubin in CCL4 treated rats may be attributed to hepatic damage which ends up in cirrhosis associated to oxidative stress where oxidation of fatty acids in hepatocytes membrane and the formation of a thick extracellular matrix are the main causes of the loss of the homeostatic equilibrium in the liver