Tobacco may have a negative effect on phagocytosis of Tetrahymena. The effect tobacco had was looked at during the experiment where tobacco was added to Tetrahymena’s food. A control solution without tobacco was used in order to be able to tell if the tobacco made something change. The tobacco ended up negatively effecting the Tetrahymena because it caused the Tetrahymena to eat less, and slower. In conclusion the tobacco did in fact have a negative effect on phagocytosis of Ietrahymena.
Introduction: Tetrahymena are ciliated protest that are found in freshwater, and are also single celled. Tetrahymena are able to live in different …show more content…
The reason why the tetrahymena ate more when there was not tobacco was because the tobacco made it harder for them to eat, by inhibiting the cilia from putting the food into their mouth. It makes since that the tobacco would inhibit it because in humans the tobacco makes breathing harder, and that’s what causes smokers to cough. My hypothesis was right because the tobacco did in fact have a negative effect on phagocytosis of Tetrahymena by causing it to eat less. Some problems that I may have encountered while doing the experiment was making sure not to kill the Tetrahymena. In order to solve that problem the slide covers had to be put on very slow and gentle. Another problem that was encountered was the Tetrahymena moving too fast while looking into the microscope. Glutaraldehyde was put in the solution to stop the Tetrahymena from moving. A problem we faced was there not being enough Tetrahymena. In order to make sure we had enough of everything, the measurements of everything that was used was cut in half. The centrifuge tubes had to stay open the whole time, and that was another problem because it was tempting to close them and put them down. Some future questions that I would have for this experiment are; how would any type of drug effect phagocytosis of Tetrahymena? Would the results of the experiment change if I had different