This experiments purpose is to study the effects of manmade chemicals on daphnia. By observing 2-3 daphnia in 4 different environments I rated each Daphnia magna on a scale of 0 to 5 on how it affected their health. I averaged each of the daphnia’s heath based on their behavior and apperence. One pure water, then three other environments exposed to antifreeze, pesticides, and cigarette ash. After observing each environment for 30 minutes I discovered that cigarette ash is the most toxic to daphnia I rated their health at a 0 and antifreeze is the only chemical I tested that didn’t result in death I rated the daphnia exposed to antifreeze at a 2 due to the change in appearance and downfall of health .…
Enzymes are protein molecules that significantly speed up the rate of virtually all of the chemical reactions that take place within the cell. In the following lab was to examine an enzyme that is found in pineapples. That enzyme is bromelain its breaks down protein into their protein into their amino acid by a process of hydrolysis. They are found in tropical areas like Hawaii, Thailand, and Brazil. For the first experiment the hypothesis was that the when gelatin was added to the water, fresh pineapple juice, and canned pineapple juice and submerged into the ice chest the solutions would turn into Jell-O.…
Aim: This is an investigation to establish the effect of different temperatures (from 0°C -100°C) on the production of oxygen gas in a potato catalase reaction, using hydrogen peroxide as a substrate. The oxygen gas produced will be measured using a gas pressure sensor. Introduction: Enzymes are proteins, known as biological catalysts that increase the rate of reaction in a biochemical reaction without being chemically altered.…
It was predicted that when the pH level increases, the rate of reaction increases. Comparing the average mass’s in Figure 1 and 2, the hypothesis is supported. In Figure 1 and looking at Data Table 1, In the duration of 10 seconds the mass only decreased by 0.1 grams, which is slower when compared to Figure 2 and Data Table 2, where the mass decreased by 0.039 grams in a span of 10 seconds. The rate of reaction in the first Figure 1, slope 1, is showcasing the initial mass of the experiment to 30 seconds. Within the first 30 seconds the mass only decreased by 0.2 grams.…
The substrate in this reaction is the H2O2. The purpose of this lab is to determine how temperature, pH, boiling, and hydroxylamine effected the activity of the enzyme, peroxidase. Temperature can change the speed with which the substrate and enzyme bind together. When temperatures are higher they move at faster speeds, which could cause the hydrogen bonds to break. The pH values can change the 3D shape of the proteins and, therefore, influence how well the substrate and enzyme bind together.…
The effect of altering temperature on the rate of liver enzyme activity is measured in this experiment to be able to visualize how temperature in the environment and in the body can manipulate the rate of enzyme activity. Enzymes are protein catalysts that lower the amount of activation energy required to start a biochemical reaction. According to Dr. Meyertholen (2015), enzymes are structured to work with certain substrates, meaning that the structure determines the function of the enzyme. If the enzymes shape is altered, they become denatured. Denaturation of an enzyme can occur with changes in factors such as temperature, pH, or salinity.…
The experiment began with a reaction of a 1cm3 cube of lamb liver and 5mL of hydrogen peroxide solution being put in test tubes in a beaker filled with 10℃ water until the temperatures of the liver and hydrogen peroxide were 10℃ also. The liver was put into a 100mL measuring cylinder and the hydrogen peroxide was also poured in as the timer was started. After twenty seconds, several millilitres of froth had been produced from the chemical concoction. This froth was recorded into a results table, and the previous steps were repeated but with increasing temperature increments of 10℃ until 50℃. After carefully completing the experiment, the final results obtained by measuring the volume of the froth produced after 20 seconds, were recorded in a table - see Table 1, and able to be observed in a graph, see Figure 7.…
The Effect of pH Level on the Amount of Brine Shrimp Hatched By: Maggie Lazaroski and Paloma Diaz Period 1, January 8 2014 Introduction Paragraph In this experiment, we will be using brine shrimp to measure the effects of pH on the survival rate of invertebrate creatures. We will use white vinegar and baking soda to alter the level of pH, the white vinegar to make the pH level higher and the baking soda to make the pH lower. We will be feeding the shrimp yeast, which is completely safe for them to be consuming.…
For this experiment my hypothesis was correct. The control petri dish germinated nine out of ten radish seeds, and the acid rain petri dish germinated zero out of ten radish seeds. For the second experiment, I said in my hypothesis was that both sets of plants, that were given the acid rain solutions, would underperform compared to the control; and the set of plants given the acid rain solution with a pH of three would underperform the control, as well as, the set of plants given and acid rain solution with a pH of five. For this experiment my hypothesis was half correct. Group B, with the acid rain solution with a pH of three, underperformed both group C, with the acid rain solution with a pH of five, and group A, the control.…
During the first lab period, The gram stain show that the unknown bacteria was a gram neative rod shaped microbe. After this step was established, this next part was to select the best tests and mediums to determine the unknown bacteria. This medium are use to test the metabolic characteristics of the unknown bacteria. In order to identify the unknown bacteria from other bacterias the following medium was used: MSA plate, SIM plate, nitrate reduction test Nutrient gelatin and Urease broth. For Metabolic tests, only Catalase test were performed on the unknown bacteria.…
In this lab we learned the basics of enzymes and how they work. We were able to perform a quantitative assay of the activity of an enzyme in a tissue extract using a spectrophotometer. Also, we had to organize the data that was provided into tables and graphs so we could have the ability to test a few hypotheses. For example, we tested the whether the rate of the reaction was influenced by enzyme concentration, whether the activity of the enzyme was influenced by temperature and also too see if the activity of the enzyme was influenced by the pH of the solution, which you will then see the results on the graphs and charts that I provided. In figure one we show results of the trial run from the enzyme activity where the absorbance was at 500nm.…
Hypothesis: If the amount of baking soda is increased then the length of its reaction with vinegar will increase because there will be more soda bicarbonate for the acetic acid to react with. Equipment: Baking Soda Vinegar 5 glasses Safety glasses Measuring Spoons Stopwatch Scale Variables: -Independent variable: baking soda (soda bicarbonate) -Dependent variable: length of reaction when baking soda and vinegar are mixed together (in seconds). -Controlled variables: vinegar (acetic acid), glass,…
Data Analysis The scientific question for this experiment is:” Does the amount of baking soda added to vinegar affect the amount of CO2 produced?” “If more baking soda is added to 50mL of vinegar, then more CO2 gas will be produced,” was the hypothesis. The independent variable was the different amounts of baking soda. Therefore, the dependent variable was the amount of CO2 produced.…
Analysis Questions: 1. The primary reaction catalyzed by catalase is the decomposition of hydrogen peroxide to form water and oxygen, which occurs spontaneously, but not at a very rapid rate. Write a balanced equation for this reaction. Label the reactant and the product. (Remember that catalase is not a reactant or a product and can be written over the arrow separating the reactant from the products.)…
Hey class, for my science fair, I have decided to do a research and experimental project on how pH values affect seed germination. In order to gain knowledge about the influences of pH values on seed germination, I decided to grow red kidney beans for 10 days with all acidic, neutral, and basic water. My hypothesis was that if multiple beans were to be grown with water containing different pH levels, then each bean plant would have different outcomes in terms of growth because depending on the pH level, the substances within the water vary and may become a harmful or a beneficial influence on the plant’s growth. I was, fortunately, correct and was able to carry out a decent test.…