Enzyme Catalyzed Reaction Lab Report

In an enzyme catalyzed reaction, it is important that we have an enzyme, a substrate, and a product. Alkaline Phosphatase, the enzyme in our experiment, is an enzyme protein found in all body tissues especially the liver, intestines, and bone (U.S. National Library of Medicine). In order for body processes to carry on in a normal and efficient way, it is critical that this enzyme is present in the system of living things. Alkaline Phosphatase is important for the development of teeth and bones as well as keeping the tissues of the liver and kidneys healthy (Genetics Home Reference). The phosphatase in the enzyme is necessary to remove phosphate groups. The substrate that we will be using is p-nitrophenyl phosphate, which will be converted into the product of p-nitrophenol plus a phosphate group, which was removed by the Alkaline Phosphate. (Steinberg, 54). …show more content…
The p-nitrophenol standard was used to create the dilutions in order to create the standard curve. By using the slope of the standard curve graph, we were able to convert our OD values we collected during the experiment into concentration values. When observing adding enzyme vs. adding no enzyme, we were to make sure that took special precautions when adding the components of each sample. Lack of addition and forgetting to blank after each sample will cause improper readings and data. While varying the amount of substrate and enzyme in the last two parts of the experiment, careful addition of each component is necessary. Also, a timely rotation of samples in and out of the spectrophotometer will speed up efficiency in collecting data. Overall efficiency of buffer, substrate, and enzyme addition as well as rotating the samples accurately and timely produces a successful enzyme catalyzed