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1447 Words Jul 29th, 2015 6 Pages
| | | | Exploring the Effects of Enzymes on Fat Using Phenol RedVeronica ChengSBI 3U0Turner05 December 2014 | |

Introduction
Enzymes are proteins which help break down chemical substances such as other proteins, carbohydrates or fats that are necessary for life (Novozymes, 2014). In this experiment, the effects of water, pancreatic juice, and trypsin solutions were observed on milk. The use of the phenol red indicator helps detect the pH level. The lower pH level will cause the colour to shift from red to orange, and possibly to yellow; if no reaction takes place, then colour will stay the same red/ pink hue.

Question
Has each of the enzymes broken down the perspective substrate (fat molecules) in milk?

Hypothesis
The
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2mL of milk solution was added into the 4 test tubes 6. Time of milk added to solution was recorded; 11:35am. 7. Solutions were then stirred by the glass rod. 8. The test tubes were placed in the hot water bath. Water was kept at a constant temperature of about 37°C by using ice cubes to cool it. 9. Observations and recording of changes took place in a table of each test tube at 2 minute intervals for 20 minutes.

Observations :
Table 1 : Milk with Enzymes Kept in Body Temperature vs. Time in Minutes Time | Test Tube A | Test Tube B | Test Tube C | Test Tube D | 11:35 | Start time/ ingredients all added at this time. | 11:38 | Placed in water bath. Dark red pink in colour; watermelon colour. | Placed in water bath. Strong, orange colour. | Placed in water bath. Strong, orange colour; lighter than B. | Placed in water bath. Strong, orange colour; lighter than B. | 11:40 | No colour change | No colour change | No colour change | No colour change | 11:42 | No colour change, nothing noticeable | No colour change, nothing noticeable | No colour change, nothing noticeable | No colour change, nothing noticeable | 11:44 | Darkened red colour | Lighter, orange colour | Lighter, orange colour | Lighter, orange-peach colour | 11:46 | Darkened to a deeper red colour | Lighter, orange-peach colour | orange-peach colour | Same | 11:48 | Same | Same | Same | Same, nothing noticeable |

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