Technical aspects of arterial hepatic artery embolization in Sprague Dawley rats for interventional radiology (IR) research
Purpose
Currently, there is an increasing interest in designing and testing new therapeutic options for the endovascular treatment of liver tumors. Different classes of targeted therapies, as well as embolic agents, and delivery systems are under development. Therefore, the characterization of preclinical models to optimize intra-arterial hepatic embolization is crucial to accelerate the development of effective treatments. The purpose of this study was to identify significant technical aspects that may influence the success rate of intra-arterial hepatic therapy embolization in a rodent model commonly used in IR research (662 characters).
Methodology …show more content…
We used an orthotopic model of liver tumors, consisting in subcapsular liver implantation of N1S1 cells. Fifty tumor bearing and forty-one non-tumor bearing Sprague Dawley rats underwent to hepatic artery catheterization via the left common carotid artery access under fluoroscopic guidance. X-ray angiograms were recorded and further analyzed to investigate anatomical structure and diameter of the proper, left and right hepatic arteries. The weight of rats was recorded at the time of angiography. Technical success of the procedure was defined as the stable placement of the microcatheter tip into the proper hepatic artery. All the procedures were conducted following the research protocol approved by the Institutional Animal Care and Use Committee. Spearman's rank correlation coefficient was calculated to determine the relationship between size of hepatic artery and weight of rats. Mann-Whitney and Wilcoxon tests were used to evaluate the differences between groups. (1029
Purpose
Currently, there is an increasing interest in designing and testing new therapeutic options for the endovascular treatment of liver tumors. Different classes of targeted therapies, as well as embolic agents, and delivery systems are under development. Therefore, the characterization of preclinical models to optimize intra-arterial hepatic embolization is crucial to accelerate the development of effective treatments. The purpose of this study was to identify significant technical aspects that may influence the success rate of intra-arterial hepatic therapy embolization in a rodent model commonly used in IR research (662 characters).
Methodology …show more content…
We used an orthotopic model of liver tumors, consisting in subcapsular liver implantation of N1S1 cells. Fifty tumor bearing and forty-one non-tumor bearing Sprague Dawley rats underwent to hepatic artery catheterization via the left common carotid artery access under fluoroscopic guidance. X-ray angiograms were recorded and further analyzed to investigate anatomical structure and diameter of the proper, left and right hepatic arteries. The weight of rats was recorded at the time of angiography. Technical success of the procedure was defined as the stable placement of the microcatheter tip into the proper hepatic artery. All the procedures were conducted following the research protocol approved by the Institutional Animal Care and Use Committee. Spearman's rank correlation coefficient was calculated to determine the relationship between size of hepatic artery and weight of rats. Mann-Whitney and Wilcoxon tests were used to evaluate the differences between groups. (1029