Boris Kozarski
Lab partner: Donovan Ortiz
9/23/16
Bio 118: A-65
GTA: Mian Li, UGTA: Shelby Schaffer
Intro: Enzymes are important biological molecules that help catalyze numerous reactions in the body. They are an arrangement of proteins designed to serve very specific roles. Enzymes contain active sites that substrates bind to. The enzymes then help orient those substrates so they achieve ideal reaction conditions. As a result, substrates are broken down much faster than they would on their own (Lawless 2016). Due to the fact that so many different arrangements of proteins are possible, it’s no surprise that so many reactions in the body are all able to proceed faster with the help of enzymes. In this lab, catalase was used to break down hydrogen peroxide and measure the reaction rates depending on the H2O2 concentration. The purpose of this was to see if the concentration of H2O2 affected the rate of the reaction. The hypothesis tested was that larger substrate concentrations should result in …show more content…
10mL of distilled water was then poured in gradually using a graduated cylinder, and the mixture was ground up using a pestle in order to extract the enzyme from the liver (Lawless 2016). The enzymes mixed with the water and an aqueous solution was formed. It was important that the enzyme solution was kept on ice to keep it cold. Three test tubes were then filled with different concentrations of H2O2; the first contained 4mL of H2O2 and 0mL of water, the second contained 2mL of H2O2 and 2mL of water and the third contained 1mL of H2O2 and 3mL of water. 250uL of enzyme solution was then added to each test tube using a micropipette (Lawless 2016), and the height of the bubble columns was measured after 10 seconds (in centimeters). The test tubes were duplicated to obtain more accurate results. The effect of reaction rate based on substrate concentration was then
Lab partner: Donovan Ortiz
9/23/16
Bio 118: A-65
GTA: Mian Li, UGTA: Shelby Schaffer
Intro: Enzymes are important biological molecules that help catalyze numerous reactions in the body. They are an arrangement of proteins designed to serve very specific roles. Enzymes contain active sites that substrates bind to. The enzymes then help orient those substrates so they achieve ideal reaction conditions. As a result, substrates are broken down much faster than they would on their own (Lawless 2016). Due to the fact that so many different arrangements of proteins are possible, it’s no surprise that so many reactions in the body are all able to proceed faster with the help of enzymes. In this lab, catalase was used to break down hydrogen peroxide and measure the reaction rates depending on the H2O2 concentration. The purpose of this was to see if the concentration of H2O2 affected the rate of the reaction. The hypothesis tested was that larger substrate concentrations should result in …show more content…
10mL of distilled water was then poured in gradually using a graduated cylinder, and the mixture was ground up using a pestle in order to extract the enzyme from the liver (Lawless 2016). The enzymes mixed with the water and an aqueous solution was formed. It was important that the enzyme solution was kept on ice to keep it cold. Three test tubes were then filled with different concentrations of H2O2; the first contained 4mL of H2O2 and 0mL of water, the second contained 2mL of H2O2 and 2mL of water and the third contained 1mL of H2O2 and 3mL of water. 250uL of enzyme solution was then added to each test tube using a micropipette (Lawless 2016), and the height of the bubble columns was measured after 10 seconds (in centimeters). The test tubes were duplicated to obtain more accurate results. The effect of reaction rate based on substrate concentration was then