Gram Positive Bacteria Report

Improved Essays
The most common bacterial infections that are gram positive are staphylococci which produce enzymes such as catalase which break down hydrogen peroxide bonds, bacillus cereus - food poisoning, bacillus anthracis - anthrax and clostridium tetani - tetanus. Penicillin is an antibiotic that prevents gram positive bacteria from forming peptidoglycan. Peptidoglycan plays a key role in the cell wall. If peptidoglycan was not present, then the bacterium would swell and burst due to the high internal pressure. Cephalosporin is another antibiotic that works against gram positive bacteria as they prevent the bacteria walls from synthesis. Sulfamethoxazole and Sulfisoxazole are antibiotics that are also effective in working against gram positive bacteria. …show more content…
This test is done to determine whether a zone of inhibition is seen. The size of the zone of inhibition will be dependent on how effective the antibiotic is at stopping the bacterium from growing. A strong antibiotic will leave a large zone of inhibition. Penicillin is an antibiotic that works against gram positiveThe gram stain technique is used to distinguish between gram positive bacteria and gram negative bacteria. This technique is used because the gram negative and gram positive bacteria both have different cells wall structures and also respond differently depending on which type of antibiotic they are given. For example, penicillin which is a type of antibiotic will prevent the synthesis of the cell wall in gram positive bacteria however will not work in gram negative bacteria. Penicillin can be used to treat many different infections such as skin infections. Penicillin is an antibiotic that works against gram positive bacteria as it prevents the peptidoglycan from being formed. Daptomycin is another antibiotic which is used against gram positive bacterial infections. This antibiotic can also be used to treat skin infections. The culture should be left in an incubator for at least 24 hours. This is done to allow the agar plate to get used to the environment and also to allow a zone of inhibition to be seen on the agar plate if the antibiotic is …show more content…
This would usually be a bacteria. These samples can then be taken from the colonies and a new microorganism can be grown on a new agar plate. When streak plating is being carried out, you need to ensure the agar plate is dry and there is no moisture present on the agar plate. This is to ensure there is no contamination which could affect the results being obtained. Once the culture has been placed into an incubator, it should be left for at least 24 hours. This is to allow the culture to get used to the environment it is in and to also allow the growth of the microorganism/bacteria. Streak plating is a very easy technique to carry out and the results are provided fairly quickly. Furthermore, it is also very easy to classify microbes using streak plating. Single bacterial or fungal species can be identified based on their morphological differences (size, shape and colour) and then can be sub-cultured to a new media plate to obtain a pure culture for further analysis. The colonies present on an agar plate can be classified depending on many different features. These are the shape, colour and even structure. Many different bacteria colonies are yellow/white in colour and have a circular shape. The different types of colony shapes are circular, irregular, rhizoid and filamentous. If there are 2 types of colonies observed then this would mean the patient has got 2 different infections and thus may

Related Documents

  • Improved Essays

    Firstly, bacteria acquire their resistant genes from their plasmid. Antibiotic resistance is when a bacteria survives against antibiotics and “learns” how to, “multiply in the presence of therapeutic levels of an antibiotic.” (www.tuff.edu) The surviving bacteria multiply to form antibiotic resistant bacterias. Thus, the cycle continues. Why does this phenomenon occur in the first place? One explanation is that when bacteria are put into a hostile environment with a selective pressure, they genetically mutate to better suit the hostile environment.…

    • 1067 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Antiseptics are used to treat and prevent infection on skin, but many antiseptic products use different active ingredients to kill or slow the growth of bacteria. Several commonly used antiseptics contain active ingredients include isopropyl alcohol, iodine, chlorohexidine gluconate, and hydrogen peroxide. The use for products with these ingredients also depends on the type of wound since certain active ingredients have different mechanisms for killing bacteria. However, a growing concern in modern medicine is how bacteria can become resistant to antimicrobial agents such as antiseptics. When bacteria is exposed to low concentrations of antimicrobial agents, some of the bacteria can survive and mutate to produce a better line of defense against…

    • 829 Words
    • 4 Pages
    Improved Essays
  • Superior Essays

    Rosacea Infection

    • 1220 Words
    • 5 Pages

    The normal strain plays a strong role in maintaining healthy skin. However, when there is a pathogenic strain, it can affect the skin tremendously. Harmful strains can induce harmless ones into becoming pathogenic through different portals of entry that are exposed, and in the skin, certain bacteria can also initiate infection. All in all, this outbreak that the patients acquired was diagnosed as a rosacea infection where the toll-like receptors 2 from a pathogenic strain of rosacea was the cause of antibiotic resistance. We used both the classical methodology and molecular methodology to identify the pathogen that caused the…

    • 1220 Words
    • 5 Pages
    Superior Essays
  • Superior Essays

    These include species such as Penicillium notatum and Penicillium cephalosporium. These are effective against gram-positive bacteria by inhibiting peptidoglycan synthesis (Todar,2012). There are a few types of Streptomyces including clavuligerus, cattleya, and griseus. These all work against both gram-positive and gram-negative bacteria. Another type of organism responsible for creating antimicrobial agents is Bacillus subtilis which inhibits wall synthesis and Bacillus polymyxa that disrupts the cell membrane (Harvey,1998).…

    • 904 Words
    • 4 Pages
    Superior Essays
  • Improved Essays

    Due to its active antibacterial agent and its unique chemical structure (C31H48O6), the Fusidic acid acts as a bacterial protein synthesis inhibitor that prevents the growth and replication of the bacteria. The spectrum of antibacterial activity is primarily effective against gram-positive bacteria, Streptococci, Coryne-bacteria, Neisseria, Moraxella, Legionella pneumophila, and Bacteroides fragilis by preventing the turnover of elongation factor G (EF-G) from the ribosome. It is inactive on most gram-negative bacteria. Fucidin is generally prescribed to treat common skin infections as impetigo contagiosa, angular cheilitis, infected dermatitis and erythrasma and secondary skin infections such as infected wounds and infected burns. The Fucidin ointment has the same effectiveness as the the cream however it contains sodium fusidate, which is the sodium salt of Fusidic acid.…

    • 973 Words
    • 4 Pages
    Improved Essays
  • Superior Essays

    They also target certain bacteria’s cell walls that differentiates them from human cells, weakening their defences by either destroying it or preventing the development of the membrane. Depending on the type of bacteria attacking the body, a patient may be prescribed a course of either narrow spectrum or broad spectrum antibiotics. Broad spectrum antibiotics target a wider variety of bacteria, for example cephalosporin which belongs to the penicillin group of antibiotics. Where as narrow spectrum antibiotics specifically target certain types of bacteria, for example Azithromyin which belongs to the macrolide antibiotic group. If the bacteria is passed on to another human, this assists in the migration of gene flow for the newly resistant genes.…

    • 1137 Words
    • 5 Pages
    Superior Essays
  • Superior Essays

    Pathogenic Bacteria

    • 1075 Words
    • 4 Pages

    It includes Mucus membrane, secretory immunoglobulin A (sIgA), macrophages and antigen presenting cells (APCs). A good example of primary innate immune defense is the mucus membrane, which eliminates bacterial adhesion by ciliary movement or mucus in the upper respiratory tract (Fedtke et al., 2004). Bordetella pertussis is a well-known example of the strategy that disables the clearance role of ciliary epithelial cells in the upper respiratory tract. This bacterium releases tracheal cytotoxin, which impairs ciliary movement and leads to the colonization of the microorganism (Hornef et al., 2002). SIgA covers the mucus membrane, and it is part of the primary defenses.…

    • 1075 Words
    • 4 Pages
    Superior Essays
  • Great Essays

    Evasion The polysaccharide capsule obtained from B. Anthracis prevents engulfment by macrophages as well as preventing opsonization of the bacterium. Toxin…

    • 1330 Words
    • 6 Pages
    Great Essays
  • Improved Essays

    Peptic Ulcer Case Study

    • 1819 Words
    • 7 Pages

    Unfourtonelty, these enzymes also produce chemicals that help protect the lining so when blocks the enzymes from producing chemicals it then weakens the defenses of the lining allowing an ulcer to form. (Crowe and Peura, D, 2014) The last cause is not as common and is due to a tumor either cancerous or non-cancerous located in the stomach, pancreas or duodenum. (Crowe and Peura, D, 2014) Common belief was that stress and spicy foods caused peptic ulcers which is false, they make the ulcer worse but do not cause it. (Crowe and Peura, D,…

    • 1819 Words
    • 7 Pages
    Improved Essays
  • Great Essays

    Triclosan Essay

    • 1687 Words
    • 7 Pages

    Bacteria are becoming resistant through three separate strategies explained by Dr. Salyers in “Revenge of the Microbes how Bacterial Resistance is Undermining the Antibiotic Miracle”. The first way bacteria try to defeat antibiotics is by turning the active drug into its inactive form. The bacteria do this by releasing specific proteins that interact with the antibiotic in a not so favorable way effectively making the drug useless. However, this method is not the most effective and is a way for the bacteria to prevent buildup of the concentration of antibiotics inside the cell. If the antibiotic does make its way inside the cell the bacteria is equipped with a protein pump in its cytoplasmic membrane.…

    • 1687 Words
    • 7 Pages
    Great Essays

Related Topics