Elizabeth Casey
Lab Partners: Cara Hull, Erin Magennis, Claire Pfeffer
Bio2300 Section 1
10/6/16
Dr. Henle
Testing Effects of Glucose and Sodium Chloride on E.coli Cell Division
Hypothesis: We hypothesized that if E.coli cells are grown in broth containing 1% 1M glucose, then they will divide more rapidly than they would without the glucose additive. Our independent variable was the glucose, and our dependent variable was E.coli cell density.
Methods: This procedure was obtained from the Growth Kinetics in Bacterial Cell Culture in the Cell Biology Lab Manual of Carthage College (2016). The wavelength of the spectrophotometer was set to 600nm. Four flasks were obtained that contained 50mL bacteria broth and labeled as follows: control …show more content…
A 1% glucose solution was found to have a positive effect on E.coli growth (Siddique and Vardo-Zalik). To optimize E.coli growth over a period of seven hours, E.coli was grown in a 1% 1M glucose bacteria broth, resulting in a 9.6mM glucose solution. As displayed in Figure 1, we found that E.coli cells cultured in bacteria broth alone grew at approximately the same rate as the E.coli grown in the broth containing the glucose additive; however, the E.coli cultured with the glucose resulted in a higher cell density/mL than the cells grown in the control flask with only the bacteria …show more content…
This finding goes against our hypothesis, as we had hypothesized that glucose would have caused greater cell growth than the cells in the control flask over the span of seven hours. The data obtained from the glucose portion of the experiment conflicts with the findings of Shehzad Siddique and Anne M. Vardo-Zalik, whose study concludes that any amount of glucose has a positive effect on E.coli cell growth. Alternatively, NaCl has been found to inhibit E.coli growth (Don, 2008). This correlates directly with our data, as we found that the population density of the E.coli cells grown in the NaCl solution was lower than the cells grown in the bacterial broth control flask. Furthermore, both the variables of the 9.6mM glucose and the 3mL 50nM NaCl resulted in graphs that fail to have an end to the growth period of the cells, indicating that the k and g values calculated for each flask may be skewed. Had the experiment continued until there was a clear end to the growth period of the cells, this information could be deemed more
Lab Partners: Cara Hull, Erin Magennis, Claire Pfeffer
Bio2300 Section 1
10/6/16
Dr. Henle
Testing Effects of Glucose and Sodium Chloride on E.coli Cell Division
Hypothesis: We hypothesized that if E.coli cells are grown in broth containing 1% 1M glucose, then they will divide more rapidly than they would without the glucose additive. Our independent variable was the glucose, and our dependent variable was E.coli cell density.
Methods: This procedure was obtained from the Growth Kinetics in Bacterial Cell Culture in the Cell Biology Lab Manual of Carthage College (2016). The wavelength of the spectrophotometer was set to 600nm. Four flasks were obtained that contained 50mL bacteria broth and labeled as follows: control …show more content…
A 1% glucose solution was found to have a positive effect on E.coli growth (Siddique and Vardo-Zalik). To optimize E.coli growth over a period of seven hours, E.coli was grown in a 1% 1M glucose bacteria broth, resulting in a 9.6mM glucose solution. As displayed in Figure 1, we found that E.coli cells cultured in bacteria broth alone grew at approximately the same rate as the E.coli grown in the broth containing the glucose additive; however, the E.coli cultured with the glucose resulted in a higher cell density/mL than the cells grown in the control flask with only the bacteria …show more content…
This finding goes against our hypothesis, as we had hypothesized that glucose would have caused greater cell growth than the cells in the control flask over the span of seven hours. The data obtained from the glucose portion of the experiment conflicts with the findings of Shehzad Siddique and Anne M. Vardo-Zalik, whose study concludes that any amount of glucose has a positive effect on E.coli cell growth. Alternatively, NaCl has been found to inhibit E.coli growth (Don, 2008). This correlates directly with our data, as we found that the population density of the E.coli cells grown in the NaCl solution was lower than the cells grown in the bacterial broth control flask. Furthermore, both the variables of the 9.6mM glucose and the 3mL 50nM NaCl resulted in graphs that fail to have an end to the growth period of the cells, indicating that the k and g values calculated for each flask may be skewed. Had the experiment continued until there was a clear end to the growth period of the cells, this information could be deemed more