Aerobic cellular respiration is the process in which energy is being released from organic compounds by chemical oxidation. In this lab we compared the change of the consumption of oxygen in germinating peas as it underwent changes in temperatures of either ten degrees Celsius, or 22 degrees Celsius (room temp.) to the oxygen consumption of dried peas as it also underwent the same temperatures of ten and 22 degrees Celsius. I hypothesized that the germinating peas would consume more oxygen than the dormant/dry peas at the 22 degree temperature rather than at the ten degree temperature because the germinating peas are supporting growing tissues and need the oxygen and proper conditions to continue to develop, whereas the dry …show more content…
In one bin make the ten degree bath by filling the bin with water and adding ice to the bath until the temperature gets down to ten degrees.
2. In the other bin make the room temperature bath by also filling the bin up with water and waiting until the water gets adjusted to the room’s temperature.
3. After the baths are at the right temperature fill the graduated cylinder with 50 ml of water. Then also add into the cylinder 15 germinating peas.
4. Record the volume of the germinating peas
5. Remove peas and place on paper towel
6. Refill the cylinder with 50 ml of water. Then place 15 dormant peas into the cylinder along with enough glass beads to get the volume to equal that of the germinating peas.
7. Remove dormant peas and glass beads and place on a separate paper towel form the germinating peas
8. Refill cylinder with 50 ml of water. Then add enough glass beads so that the volume equals that of the germinating peas.
9. Remove beads and place on their own paper towel
10. Repeat steps one-nine, this is the second set of peas and beads to be used in the room temperature bath
11. In the bottom of each of the six vials place an absorbent cotton ball
12. Place two-three drops of 15% KOH on each cotton ball without getting KOH on the sides of the …show more content…
This tells us that the germinating peas in the ten degree bath and in the room temperature bath were both using the oxygen to make glucose to support their growing cells/tissues, while both sets of dry peas weren’t consuming any oxygen; the water was being pushed out of the pipette instead. The rate of oxygen consumption for the germinating peas was higher in the room temperature bath (0.0413) than the rate of oxygen consumption in the ten degree bath (0.013). The results for the dry peas were the same, the rate of consumption for the room temperature (-0.013) was higher than the rate of consumption for dry peas in the ten degree bath (-0.0033). Our data could have been skewed by some extraneous variables such as not washing our hands when dealing with the peas and beads. This could have caused the peas or beads to have a higher rate of consumption than normal, because we could have also been accounting for the bacteria from our hands that could have also been taking in the oxygen along with the peas. Another confounding variable could have been that the pipette wasn’t tight enough in the stopper so some of the oxygen could have come out through the stopper and no the