One such method is to use the ratio of the absorbance at the λmax of nucleic acids to that of the absorbance at the λmax of proteins. If the isolated genomic DNA is relatively pure, then the A260/A280 ratio should be approximately around the range of 1.6 to 1.9. Based upon completion of the calculations, the A260/A280 ratio was found to be 1.877 (Table 1) indicating that the isolated genomic DNA is pure. However, not only can the absorbance max be used to measure the purity of the nucleic acids, but it can also be used to measure the concentration of genomic DNA. Using the calculation DNA (µg) = A260 x 50 µg/(1 A260 x 1 ml) x dilution factor x total sample volume (ml), the concentration of DNA was calculated to be 25
One such method is to use the ratio of the absorbance at the λmax of nucleic acids to that of the absorbance at the λmax of proteins. If the isolated genomic DNA is relatively pure, then the A260/A280 ratio should be approximately around the range of 1.6 to 1.9. Based upon completion of the calculations, the A260/A280 ratio was found to be 1.877 (Table 1) indicating that the isolated genomic DNA is pure. However, not only can the absorbance max be used to measure the purity of the nucleic acids, but it can also be used to measure the concentration of genomic DNA. Using the calculation DNA (µg) = A260 x 50 µg/(1 A260 x 1 ml) x dilution factor x total sample volume (ml), the concentration of DNA was calculated to be 25