Fungi Lab Report
Cellobiase (β-glucosidase) is a well characterized enzyme of the cellulose enzyme system which acts synergistically to degrade cellulose into glucose subunits (Ban152). The purpose of this experiment was to measure the rate of cellobiase activity in various club fungi types. The types of club fungi experimentally used was Agaricus bisporus (button mushroom), Pleurotus ostreatus (oyster mushroom), and Lentinula edodes (shiitake mushroom). The living environments of these three mushrooms are distinctly different. Button mushrooms are natives to grassland areas, oyster mushrooms are usually found on decaying trees and wood in clusters, and shiitake mushrooms are found on decaying trees. The hypothesis formulated was that the oyster mushrooms would have the highest rate of reaction, in comparison to shitake and button mushrooms, because it is found on decaying logs and it is larger in …show more content…
The button mushroom had an absorbance rate of .0097 absorbance/min. Lastly, the oyster mushroom had the highest rate of reaction at .0328 absorbance/min. As the time increased the rate of absorbance increase, for example (Figure 1), there was a drastic increase with the rate of reaction of the shiitake mushroom from one minute to 6 minutes.
Figure 1. The rate of cellobiase activity measured, in three different fungi types, in absorbance. The reaction rate was measure at 1,2,3,4 and 6 minute increments and the absorbance rate was measure by a SpectroVis Plus spectrophotometer. The slope of the line (absorbance/minute) indicated the rate of cellobiase