Fruit Fly Genetic Lab: Fruit Fly Genetics Lab

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Register to read the introduction… Then take one fly of a different strain, of the opposite gender. Put both flies into a vial with the growth medium at the bottom (every new vial should have the growth medium) and put the cotton plug on top (always anesthetize the flies when moving the flies in and out of their vial to avoid escapees). When it is seen that the flies have produce larvae that have hatch, kill of the parents. When the larvae have developed into adults, take the out of the vial and observe under the microscope in a petri dish. Their count how many flies there are according to phenotype and gender (use paint brush to move flies gently without squishing them).This is the F1 generation. Record all data. Then select one female and one male to put in new vial (kill of rest). Wait for larvae in the newest vial to appear (then kill of parents) and wait again for these flies to fully mature, this is the F2 generation. Move these flies to the petri dish and look under the microscope. Once again sort the flies by phenotype and gender. Record all Data. Kill of all remaining flies and clean the vials. Repeat all steps for all crosses.

Results (Data)
In this experiment it was expected that all traits exhibited in the Wild stain of the fly is dominant over the white eye strain, Ebony body stain, and vestigial wings strain no matter the gender combination. So in this experiment it was expected that for all crosses the outcome will be the F1generation having 100% Wild strain, and the F2 generation having 75% Wild stain and 25% of the other strain no matter the gender combination. The Punnet Square that will describe for all the crosses would be as the below:
Parent Cross,
Offspring is
…show more content…
And 100% of all males would have white eyes. This is congruent with the observed results: 588 Male white, and 601 Female wild (no male wild or female white). The F2 generation for this cross would be as below:

Wild ♀ X White ♂
F2 Generation

Original Parents:
Wild ♂ X White ♀ x y X
X x
X y x x x x y

The result for the F2 generation would be: 50% of female white, 50% of female wild, and 50% of male white and 50% of male wild. This is congruent with the results: 345 white males, 299 white females, 287 wild males, 290 wild females.
The below will be the Punnet Square represented the White ♂ x Wild ♀ cross:
White ♂ x Wild ♀
F1 Generation x y
X
X x
X y
X
X x
X y

In the F1 generation there are 100% of females wild and 100% of males wild. This is congruent with results: 612 wild males, 569 wild females. The next Punnet Square will represent the F2 generation of this cross:

Wild ♂ x Wild ♀
F2 Generation
Original Parents:
White ♂ x Wild ♀

X y X
X X
X y x X x
x

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