Mitochondria Experiment

Great Essays
The mitochondrion is an organelle which produces ATP or energy for all cells. The ATP produced by the mitochondria can be used to measure certain physical activities of fruit flies.
In this experiment the development, climbing, flight, and in vitro activity of Drosophilia Melanogaster flies were tested. Three different strands of flies were used. One strand was the wild-type (wt) fly, which had normal mitochondria without any mutations. Another stand was the W501 strain (hybrid) which had its mitochondria removed and replaced with that of D.simulans mitochondria. The third strand of fly used was the sdhBEY12081 strain (mutant) that had a mutation present on the sdhb gene of the mitochondria. The sdhBEY12081 gene is responsible for producing the enzyme succinate dehydrogenase, which is used in the electron transport chain for ATP production. The results showed that the sdhb (mutant) strain of flies developed the quickest, the wild-type strain was second, while the W501 (hybrid) strain had the slowest developmental time. As regards climbing ability, the wild-type strain of flies had the best climbing ability, the W501 (hybrid) strain was second, while the sdhBEY12081 (mutant) strain was last. Experiments on the flying ability showed that the hybrid strain of flies had the best flying ability, the
…show more content…
One strain of fly was the wild-type (wt). The wild-type strain had no mutations present in its genome. A second strain of fly used known as the W501 (hybrid) strain was used which did not have Drosophilia Melanogaster mitochondria, but rather D stimulans mitochondria. Finally, a sdhBEY12081 strain was used which contained the mitochondria from Drosophila Melanogaster with a mutation in the sdhb gene which codes for succinate dehydrogenase. All three strains of flies were subjected to tests, displaying their ability to fly, ability to climb, succinate dehydrogenase activity, and developmental

Related Documents

  • Improved Essays

    The glass vial containing the culture medium, pupa, and larva was removed and quickly replaced with the sponge cap soaked in ether. The adult flies were now in the Drosophila Anesthetizer. After the flies fell to the bottom of the container, the ether soaked sponge cap was replaced with a clean sponge…

    • 731 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Hot Cheeto Research Paper

    • 363 Words
    • 2 Pages

    The posterior scutellar bristles in D. melanogaster are two long bristles attached to the end of the scutellum and projected in an outward V-shape. Yet, in hot cheeto, the V-shape projection is not present because the short bristles curled to opposite sides of the scutellum. Humeral bristles, dorsocentral bristles, notopleural bristles, anterior scutellar bristles, orbital bristles, and ocellar bristles are all affected by this mutation. This mutation affects the phenotype equally in both female and male mutants. However, the mortality rate in hot cheeto females was greater than males.…

    • 363 Words
    • 2 Pages
    Improved Essays
  • Improved Essays

    Cameron Croghan, Shelby Robinett Mr. Cmaylo AP Biology 10-19-15 Energy Transfer Energy transfer occurs in all cellular activities. For three of the following five processes involving energy transfer, explain how each functions in the cell and give an example. Explain how ATP is involved in each example. Adenosine Triphosphate, or ATP, is the most useful form of energy in the human body. Humans use it for everything from lifting a pin to running, to breathing.…

    • 840 Words
    • 4 Pages
    Improved Essays
  • Decent Essays

    As a group we have taken two sterile plastic vials and placed a cup of Drosophila medium powder into each one. After adding a cup of the medium with added half a cup of distillated water into the plastic vial, which allowed the medium to dissolve into it. We allowed a few minutes for the media to be absorbed and afterwards we applied a cotton plug to close the entrance to the vial. Anesthetizing the Drosophila flies In the lab, we had the fruit flies already asleep in a green bucket filled all the way up with ice.…

    • 348 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    We hypothesized that curly wing flies would have difficulty courting past the flapping of its wings to create a “love song”. The FraxE flies are model organisms of a mutation that causes mental retardation and various behavioral problems. We did not expect very much courtship at all in this group. Lilli flies were similar to the FraxE model except they had the protein that was lost with the mutation placed roughly 50% back into them. We thought the flies with this mutation would have more courtship then the FraxE but less than the curly wing fly.…

    • 977 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    The simple but revolutionary organism Drosophila melanogaster has intricate properties that are studied to find its relations with human genes. Not only is D. melanogaster a model organism for its rapid growth, inexpensive culturing, and easy modifications, the Drosophila can provide more in-depth scientific analysis that can solve human diseases. The model organism D. melanogaster is studied to find ground-breaking research in neurology, sleep, cancer, and drug discovery. The multivariable effects in cancer can be studied by observing D. melanogaster because of its tumorigenesis caused by genes and its surrounding environment. The development of tumors in D. melanogaster is similar to the pathway of a tumor in a mammalian cell as it undergoes…

    • 1018 Words
    • 5 Pages
    Improved Essays
  • Decent Essays

    There are many organelles in the animal cell. There is the nucleus, endoplasmic reticulum, cell membrane, vacuoles, lysosomes, mitochondria, cytoplasm, ribosomes, & golgi apparatus. The nucleus directs all the cells activities,including reproduction. Endoplasmic Reticulum is a network of membranous tubules within the cytoplasm continuous with the nuclear membrane;It usually has ribosomes attached The cell membrane protects the cell and regulates what substances can enter and leave. Some animal cells have vacuoles that store food, water, & waste.…

    • 238 Words
    • 1 Pages
    Decent Essays
  • Improved Essays

    Thus, the goal of this experiment is to determine if the functional activity of mitochondria is in anyway compromised in…

    • 402 Words
    • 2 Pages
    Improved Essays
  • Improved Essays

    Support the Endosymbiotic Theory Gianna Melendez Chamberlain College of Nursing Support the Endosymbiotic Theory The endosymbiotic theory proposes that eukaryotes formed from the union of small aerobic prokaryotes with larger anaerobic prokaryotes, which form organelles. The smaller prokaryotes were not destroyed by the larger cells, but instead became internal parasites that remained surrounded by a vesicular membrane of the host. The theory proposed that mitochondria are the result of endocytosis of aerobic bacteria and chloroplasts are the result of endocytosis of photosynthetic bacteria.…

    • 1762 Words
    • 8 Pages
    Improved Essays
  • Decent Essays

    The joint evolutionary histories of Wolbachia and mitochondria in Hypolimnas bolina, published by BMC Evolutionary Biology is a great example of how the interactions of two organisms can lead to evolutionary change. This research article examines the history of the parasitic relationship between the Wolbachia, an intracellular bacteria, and the Blue Moon butterfly (Hypolimnas bolina). What the article explain is how wBol1, a particular strain of the bacteria, would infect H. bolina populations on islands throughout the pacific killing of the males of the population. The females of the population would not be affected however, they acted as a carrier, infecting the males during intercourse. Luckily, some of the males of H. bolina possessed suppressor genes which prevented their deaths, allowing the population to restore its sex ratio.…

    • 412 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    Cellular Catabolism

    • 1480 Words
    • 6 Pages

    iv. Increased enzymatic capacities for substrate catabolism v. Higher degree of clustering of subsarcolemmal mitochondria adjacent to capillaries-> higher O2 flux rates vi. Mitochondrial volume densities and surface areas per unit mitochondrial volume are near maximum theoretical limits in hummingbirds and higher than mammals-> achieved due to higher oxygen delivery capacities and substrate catabolism (Suarez et al. 1991) 5. Metabolic Substrate Turnover a. Questions i. Do hummingbirds metabolize sugars that they are readily taking in or that have been previously stored?…

    • 1480 Words
    • 6 Pages
    Improved Essays
  • Decent Essays

    Mitochondria play a very important role in the body. They create energy for the body, which is used to sustain and support growth and other functions. When the Mitochondria become diseased, the results can be disastrous. Take Mitochondrial Disease, for example. It's an inherited chronic illness that effects the mitochondria and weakens their ability to produce energy for the cell.…

    • 102 Words
    • 1 Pages
    Decent Essays
  • Superior Essays

    Succinate Dehydrogenase

    • 1659 Words
    • 7 Pages

    Question and Hypothesis Experiment I Eukaryotic cells contain several membrane-bound organelles. Plant cells are a specific type of eukaryote that are both aerobic and photosynthetic. Because of this, they contain mitochondria that serve a vital role in aerobic respiration that follows photosynthesis. The enzyme succinate dehydrogenase (SDH) is an enzyme associated with the Citric Acid Cycle that takes place in the mitochondrial matrix. SDH serves as a catalyst for a reaction in which succinate and FAD become fumarate and FADH2.…

    • 1659 Words
    • 7 Pages
    Superior Essays
  • Improved Essays

    They sequenced the genome of the cell multiple times which showed no signs of mitochondrial DNA. It also lacked any evidence of the necessary proteins in the cell which would make the mitochondria inert. They hypothesized that the cell did not have any use for the mitochondria because of its…

    • 841 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Apterous Gene Mutation

    • 714 Words
    • 3 Pages

    The apterous gene mutations is a recessive trait that is inherited causing a varying phenotypes such as different wing deformities. Other phenotypes observed are the decrease in life span and female sterility. Ap is required to regulate gene expression needed for the development of Drosophila wings and haltere discs. Decreased ap activity due to the overexpression of dLMO results in wing mutations. The phenotypes displayed in apterous mutants are places them at a disadvantage compared to wild type flies.…

    • 714 Words
    • 3 Pages
    Improved Essays