Fifty crayfish were taken to aquatic health and diseases of Faculty of Veterinary Medicine of Shiraz University. For this purpose, specimens of crayfish with symptoms such as black plaques, abscess, shell softness, corneal anomalies and leeches on the surface were selected (Fig. 1), After washing the body surface with a physiological serum using a sterile syringe from the fluid in the abscess, and also using sterile loops, the contents of the black plaques on the body surface were sampled. The samples then were transferred to the broth hart infusion broth (BHI) for bacteria and sabouraud dextrose agar (SDA) for the fungus. After 48 hours of storage at 25 ° C, to purify, the colonies were grown on culture media again passage were
