FTIR Analysis Of Cassia Handla

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FTIR analysis of the stem bark extracts of Cassia fistula has absorption bands and the wave numbers (cm-1) of the prominent peaks obtained were described in Table 2. The peak at a frequency of 3463 cm-1, 1618 cm-1, 1448.3 cm-1 and 1241.9 cm-1 were strong while the others vary from medium to weak.

Discussion: Phytochemical studies revealed the presence of secondary metabolites like tannins, flavonoid, carbohydrate, saponins, glycosides and phenol in the leaf extract while tannins, flavonoids, carbohydrate, saponins, glycosides, phenol and terpenoid in the stem bark . The presence of secondary metabolites in plants, produce some biological activity in man and animals and it is responsible for their use as herbs5 and therefore explains
…show more content…
The phytochemical analysis conducted on Cassia fistula revealed the presence of secondary metabolites like tannins, flavonoid, carbohydrate, saponins, glycosides and phenol in the leaf extract while tannins, flavonoids, carbohydrate, saponins, glycosides, phenol and terpenoid in the stem bark. Interestingly, FTIR analysis between 400 – 4000 cm-1 range showed compound with characteristic absorption bands at 3432 cm-1 for alkyne group, 1614 cm-1 for olefin and 1513 cm-1 for arene , 1240 cm-1 for alcohol and phenol, 1159 and 1112 cm-1 for ether group, 1448 cm-1 for alkanes, 567 cm-1 for alkyl bromide which were closely similar to Catechin. No other visible peaks were observed in the spectra which indicate that a simple soxhlet extraction itself yields Catechin with fewer …show more content…
Marjorie, M.C. (1999), ClinMicrobiol., 12(4): 564-582.
7. Mamta Saxena, Jyoti Saxena, Rajeev Nema, Dharmendra Singh and Abhishek Gupta (2013), Journal of Pharmacognosy and Phytochemistry; 1(6):168-182.
8. R.Ashokkumar and M. Ramaswamy(2014), Int.J.Curr.Microbiol.App.Sci 3(1): 395- 406.
9. Suman Kumar Ratnampally and Venkateshwar Chinna (2017), Int. J. Curr. Microbiol. App.Sci 6(4): 1073-1079.
10. O. N. Maitera and I.B. Chukkol (2016), World Journal of Research and Review (WJRR), ISSN:2455-3956, 3(3): 25-29.
11. J. Indhumathy, S. Gurupavitra, K. Ravishankar And A. Jayachitra (2014), Mintage journal of Pharmaceutical and Medical Sciences 3(3): 20-25.
12. Anusha Kulkarni, M. Govindappa, Channabasava1, CP Chandrappa, YL Ramachandra and Prasad S. Koka(2015), Advancement in Medicinal Plant Research ISSN: 2354- 2152, 3(1): 8-17.
13. G.Chen, R. J., Xu, G. Y., Wang, H. Y., Xu H. Z. and Liu, Y. M. (2001), Chemistry Bulletin/HuaxueTongbao, 64(6): 372-374.
14. Obdoni BO and Ochuko PO, (2001), Glob J Pure Appl Sci, 8b: 203-208.
15. GJ Kaur and DS Arora (2009), BMC Compl Altern Med, 9: 30.
16. P. Khare (2007), Indian Med Plants, Springer, 128.
17. V. Prasanth Kumar, N.S. Chouhan, H. Padh and M. Rajani (2006), J. Ethanopharm; 107,

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