Experiments In Quadrant B Experiment

Improved Essays
Results:
The data gathered displays that there were 51 colonies in quadrant A (the handle of the paper dispenser in the women’s bathroom), most were circular in shape, white in color, and smooth over all. There were 87 colonies in quadrant B (the spatula used in the sandwich bar), most being circular, white, and smooth. There were 13 colonies in quadrant C (the outer handle of the front entrance to the school); they were mostly circular, beige and shiny. Lastly, there were 55 colonies in quadrant D (the microphone part of the podium); they were mostly circular and white, all being either shiny or smooth.

Discussion:
The findings of the experiment reject my initial hypothesis that the most frequented location (the handle of the front door
…show more content…
However, they may just have not gotten to clean the spatulas from lunch the day before since it was still very early. Also, the spatula that I chose was put in the same bin that the new meat was laid out in, so I assume that a good amount of the micro organisms from the meat bin could have been on the spatula and therefore picked up with the swab. I was expecting the microphone to have quite a bit of micro organisms on it, however, due to the fact that it usually might not get cleaned as often, and because while people talk into it they tend to spit a lot and sweat when the grip the microphone holder, further spreading countless microorganisms in their mouth and on their body to the holder. The next quadrant, quadrant C, had fewer colonies than quadrants B and D, and for good reason. After the women in the bathroom use the toilet, they wash their hands before going to get paper from the dispenser, and holding the handle. Therefore, it is expected that the soap killed many of the microorganisms before they could get to the paper dispenser. Finally, quadrant A had the least amount of colonies, although the colonies were very large in size and spread out.
The possible sources of error in this experiment could have been too much exposure of the growth medium (agar)

Related Documents

  • Improved Essays

    Pinto beans or in other words common beans are the most abundant beans in both Americans, making it major source of daily nutrients for large number of civilians. This experiment was done to examine if the number of seeds used in a specific area would affect the growth process using two pots (Control, Experiment). Control pot had two Pinto beans, while the Experiment pot had four seeds. Both pots were placed in the same room with same amount of water supply and light.…

    • 1080 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    1. (4 points) The results below were obtained from a use-dilution test of two disinfectants. Cultures were inoculated into tubes with varying concentrations of disinfectants and incubated for 24 hr at 20°C, then subculture in nutrient media without disinfectants. (+ = growth; - = no growth) Disinfectant 1 Disinfectant 2 Concentration Initial Subculture Initial Subculture…

    • 1262 Words
    • 6 Pages
    Improved Essays
  • Decent Essays

    Contamination levels would vary with hospital location, medical rehabilitation ward has higher levels than other wards. Finally, simple hand washing before patient care, without hand antisepsis, is also associated with higher colony counts another important issue for medical personnel is also…

    • 42 Words
    • 1 Pages
    Decent Essays
  • Superior Essays

    Essay On Nitrate Test

    • 1176 Words
    • 5 Pages

    During the phase and process of the bacterial unknown project, vast amounts of test were performed in order to determine an unknown species of bacteria. Within the many procedures of identifying the unknown bacteria, I was first given a tube with the unknown bacteria, from which later on I started subcultures by streaking bacteria from the original tube onto a tryptic soy agar (TSA) slant. Throughout the process of identifying the unknown, new subcultures were produced in order to use the newest bacteria to conduct the tests. Thus, this process allowed the unknown bacteria to grow and maintain. Before a series of test were conducted, first a gram stain test was performed in order to determine the unknown’s gram reaction and its cell morphology.…

    • 1176 Words
    • 5 Pages
    Superior Essays
  • Improved Essays

    The first step was to streak the bacterium with a sterilized look onto a Mueller Hinten plate. The MH plate was incubated at 37 degrees Celsius for 48 hours. Upon return and observation, the MH plate did not isolate the colony enough to be able to test the bacterium. The professor then handed an alternate 10 that was a pure culture to streak. It was with a sterilized loop that some of the pure culture was taken out and gram stained as directed by the laboratory manual (1).…

    • 1057 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Throughout the semester in the microbiology lab we have been taught how to conduct multiple experiments to identify microorganisms. Many of these experiments can be used on all different types of microorganisms but cumulatively they can help isolate a singular microorganism’s identity. At the beginning of the semester we were divided into several groups and were assigned an unknown of which we were tasked with identifying. Being assigned number 14 as our unknown, my group consisting of Chijindu Eke, Austin Dicus and myself conducted these experiments over the course of the semester and have successfully identified our microorganism to be Enterobacter Aerogenes. The plan to identify the microorganism was to grow a pure and uncontaminated…

    • 1097 Words
    • 5 Pages
    Improved Essays
  • Superior Essays

    Unknown Microorganism

    • 1849 Words
    • 8 Pages

    The purpose of performing physiological tests is to figure out certain characteristics of microorganisms or to reach a conclusion on what an unknown microorganism is. It can be also used to determine what is needed for bacteria to be cultured in or on certain media. Culture media can either be liquid or solid, and they can be used to identify certain characteristics of different bacterial species. This is indicated by the growth or lack of growth in or on certain media. Solid media can also be used to isolate organisms and provide a means to observe their morphology (Wiley 55).…

    • 1849 Words
    • 8 Pages
    Superior Essays
  • Improved Essays

    The Wisconsin Fast Plant

    • 490 Words
    • 2 Pages

    In order to properly grow, plants need certain macronutrients and micronutrients. They need the macronutrients: nitrogen, phosphorous, potassium, calcium, sulfur, magnesium; and the micronutrients: boron, chlorine, manganese, iron, zinc, copper, molybdenum, and nickel. (3). Fertilizer enhances the growth rate of plants. Fertilizer adds nutrients and modifies the water retention and aeration of plants.…

    • 490 Words
    • 2 Pages
    Improved Essays
  • Improved Essays

    She then used the antibacterial soap to clean the upholstery and the view box for the x-rays. When she finish disinfecting the unit she returned to the sink and washed her rubber cleaning gloves for three time. Afterwards, she removed she rubber cleaning gloves and place them on a paper towel to store them in the bottom of the drawer in the mobile cart. The senior dental hygiene student returned to the sink to rewash her hands. She followed the hand washing rule to the letter each time, since as stated in Wilkins “Handwashing in…

    • 1434 Words
    • 6 Pages
    Improved Essays
  • Great Essays

    Infection Prevention: Hand Hygiene Kayla M. Fleming Milwaukee Area Technical College NRSAD 114: Management and Professional Concepts October 16th, 2016 Infection Prevention: Hand Hygiene It’s hard to imagine washing or sanitizing your hands every time you touch someone you’ve known for a very long time. Hand hygiene seems common place in hospital settings but may sometimes go lacking in long term care settings due simply to familiarity with the people being cared for. Regardless of the setting, the Clinical practice guidelines are the same. The purpose of this paper will be to explore the Long Term Care National Patient Safety Goal of Infection Prevention: Hand Hygiene, the Evidence-based guideline related to the National Patient Safety…

    • 1511 Words
    • 7 Pages
    Great Essays
  • Great Essays

    Interpretation and Analysis Gibberellins are a tetracyclic diterpenoid compound that stimulates the reorganization of cellular organelles and the synthesis of new proteins such as enzymes. GA exerts its influence in two manners, first by increasing the growth potential of the embryo and second by inducing hydrolytic enzymes. Once a plant releases gibberellins, its cells begin a process of elongation. Since plants are composed of single cells stacked on top of one another, this elongation of thousands of individual cells results in the overall growth of the plant. Usually in germination, the breakdown of starch to glucose in the endosperm begins shortly after the seed is exposed to water.…

    • 1348 Words
    • 6 Pages
    Great Essays
  • Decent Essays

    Mitigation Steps

    • 456 Words
    • 2 Pages

    iv. To prevent similar incidence from re-occurring, what mitigation steps will you suggest? There are a lot of mitigation steps to prevent the similar incident from re-occurring. The first one is by improving the areas of infection control, written protocols, and appropriate training in the sterile technique to ensure a good practice.…

    • 456 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    Pre-Op Job Essay

    • 740 Words
    • 3 Pages

    I could say that I have an interesting job. All though it’s a really stressing job I guess it’s not as bad as being a line worker. When I took this job I did not know all the responsibilities that came with it. There’s a lot of paper work that comes along with this job.…

    • 740 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    The lab instructor issued out a test tube labeled with letter ‘I’, which consisted of two unknown bacteria, Gram-positive or Gram-negative that were streaked from a pure culture. Sterile techniques were followed while performing precise instructions as stated in the referenced Laboratory Manual. Example 1: The first procedure performed was done by isolating a pure culture from the mixture onto a solid Trypticase soy agar (TSA) media. Sterile technique was done by flaming the loop until it turned red to ensure that there were no current bacteria on the loop avoiding contamination followed by rapidly flaming the neck of the test tube to prevent the entry and contamination of unwanted microbes.…

    • 416 Words
    • 2 Pages
    Improved Essays
  • Superior Essays

    Kaitlyn Staub Mr. G Biology Honors 4 January 2016 Title The Effect of Different Music Genres on the Growth of the Bacteria Serratia marcescens Introduction Exploring the question of does music affect bacterial growth requires much research. The purpose of answering this question is to figure out how to slow down bacterial growth by music.…

    • 1215 Words
    • 5 Pages
    Superior Essays