A. Description of the Study Area
The experimentation of the study will be conducted in PLM College of Science Laboratory at Pamantasan ng Lungsod ng Maynila, Gusaling Lacson, 6th floor. The University is located beside Department of Labor and Employment building in Intramuros, Manila, known as the “Walled City”. At the back of the University is the Philippine Red Cross - Manila Chapter. The Lacson Building where the laboratory is located is in between the University Gymnasium and Justo Albert Auditorium. The laboratory consists of several laboratory apparatus and most especially microscopes, which is greatly needed for the experimentation. Beverage samples will be collected from University Canteen, located in the University Activity Center, near the Gusaling Villegas Right Wing and PLM Pride Hall.
B. …show more content…
Triple Sugar Iron Test In triple sugar iron slant, the protocol of Acumedia Manufacturers Inc. (2010) will be used. A loopful of isolate will be inoculated. It will be stabbed into the slants to the bottom and will be streaked at the surface of the agar slants. The slants will be incubated for at least 18 to 24 hours at 37°C, and the results will be recorded afterwards.
4. Oxidation-Fermentation Test (OF Test) In OF slants, a loopful of culture will be stabbed halfway to the bottom of the slants. One slant will be placed with mineral oil to create an anaerobic environment. The OF slants will be incubated for 48 hours at 35°C, and the results will be recorded and photographed.
5. Methyl Red and Voges-Proskauer Test
A pure culture will be inoculated in a Methyl Red-Voges Proskauer Broth tubes. The tubes will be then incubated at 35°C for 48 hours. One tube will be placed with five drops of methyl red reagent, and the other with Barrit’s reagent. Methyl Red test is used for the indication of fermenting ability of bacteria, producing mixed acid. Voges-Proskauer test is used to detect the production of acetoin, also known as 2,3-butanediol.
6. Indole – Motility
The experimentation of the study will be conducted in PLM College of Science Laboratory at Pamantasan ng Lungsod ng Maynila, Gusaling Lacson, 6th floor. The University is located beside Department of Labor and Employment building in Intramuros, Manila, known as the “Walled City”. At the back of the University is the Philippine Red Cross - Manila Chapter. The Lacson Building where the laboratory is located is in between the University Gymnasium and Justo Albert Auditorium. The laboratory consists of several laboratory apparatus and most especially microscopes, which is greatly needed for the experimentation. Beverage samples will be collected from University Canteen, located in the University Activity Center, near the Gusaling Villegas Right Wing and PLM Pride Hall.
B. …show more content…
Triple Sugar Iron Test In triple sugar iron slant, the protocol of Acumedia Manufacturers Inc. (2010) will be used. A loopful of isolate will be inoculated. It will be stabbed into the slants to the bottom and will be streaked at the surface of the agar slants. The slants will be incubated for at least 18 to 24 hours at 37°C, and the results will be recorded afterwards.
4. Oxidation-Fermentation Test (OF Test) In OF slants, a loopful of culture will be stabbed halfway to the bottom of the slants. One slant will be placed with mineral oil to create an anaerobic environment. The OF slants will be incubated for 48 hours at 35°C, and the results will be recorded and photographed.
5. Methyl Red and Voges-Proskauer Test
A pure culture will be inoculated in a Methyl Red-Voges Proskauer Broth tubes. The tubes will be then incubated at 35°C for 48 hours. One tube will be placed with five drops of methyl red reagent, and the other with Barrit’s reagent. Methyl Red test is used for the indication of fermenting ability of bacteria, producing mixed acid. Voges-Proskauer test is used to detect the production of acetoin, also known as 2,3-butanediol.
6. Indole – Motility