Exchange Chromatography Lab

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Chromatography is a key aspect in chemistry. It allows one to separate molecules based on a variety of things. In labs 3 and 4 we looked at two of the characteristics that molecules carry that can help separate them. Lab 3 used spectrophotometry once again, but the concept that garnered the most interest was ion exchange chromatography. Ion exchange chromatography works by separating molecules based on the individual charges they carry. In our case we used anion exchange chromatography, which means that our resin was positively charged so the negative molecules would interact with the column. On the other hand the positive molecules will move straight through the column. The pH used during the experiment affected this system though, but this

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