Essay on Examination Questions On Glucose Fermented

792 Words 4 Pages
Unknown #30
1a. Glucose fermented. 2a. Sulfur reduced. 3a. Lactose fermented…………………………………………Citrobacter freundii 3b. Lactose not fermented 4a. Sucrose fermented…………………………………….Proteus vulgaris 4b. Sucrose not fermented………………………………Proteus mirabilis 2b. Sulfur not reduced. 5a. Lactose fermented. 6a. Sucrose fermented. 7a. Ornithine decarboxylase present……Enterobacter aerogenes 7b. Ornithine decarboxylase not present…….Klebsiella pneumonia 6b. Sucrose not fermented……………………………….Escherichia coli 5b. Lactose not fermented…………………………………….Serratia marcescens
1b. Glucose not fermented 8a. Glucose oxidized…………………………….........…………Pseudomonas aeruginosa 8b. Glucose not oxidized…………………………………………......Alcaligenes faecalis

Test Question being asked Observations Interpretations
Gram Statin Is the unknown gram positive or gram negative? The unknown was red and rod shaped The unknown is Gram negative bacilli
TSI Can the unknown ferment glucose, reduce sulfur, and produce gas? The unknown had a yellow slant and a yellow/black butt. A/H2S The unknown can ferment glucose and at least one other sugar and it can reduce sulfur.
Phenol Red Is glucose fermented? Is lactose fermented? Is sucrose fermented? Glucose: yellow
Lactose: yellow
Sucrose: red The unknown ferments glucose and lactose, but does not ferment sucrose

Testing Unknown #30
The first test that I did for my unknown was a gram stain. A gram stain is a test designed to differentiate…

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