Biofuels are organic chemicals that can be used as an environmentally friendly alternative to fossil fuels which are known to produce greenhouse emissions. Corn sugar is used in the production of these biofuels, the most prevalent of which is ethanol (Ethanol Biofuel). Although ethanol shows great potential as a fossil fuel alternative, there isn’t enough corn sugar around to produce ethanol for all machinery. Also, too much fossil fuel is involved in its production to provide any significant economic growth. Many experts believe that a better way to produce ethanol will be from the cellulose in grasses and saplings (Biofuels Facts, 1). If cellulose can be used instead of corn sugar, it could be more efficient and emit less greenhouse gases.
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From Table 1, it may be seen that an increase in the concentration of the inhibitor in portabella changes the Km from 0.33 to 1.6mM but presents no change in Vmax. It may also be seen from Table 1 that an increase in the concentration of the inhibitor in oyster increases the Km from 0.72 to 1.8mM and also presents a slight increase in Vmax from 3.82 to 4.07nmol/min. When no inhibition is added to the substrates, there is substantial difference between the Portabella and Oyster Km values, Portabella being 0.33mM and Oyster being 0.72mM. With 10mM of glucose, the difference still remains with Portabella being 1.60mM and Oyster being 1.80mM. Oyster mushroom also has a higher Vmax than Portabella with or without glucose inhibition, Oyster being 3.82nmol/min and Portabella 2.21nmol/min without inhibition and Oyster being 4.07nmol/min and Portabella 2.21nmol/min with 10mM of glucosidal inhibition. All the data stated above may be found illustrated as …show more content…
Because the Km value for the Oyster extract is significantly larger, this means that the Oyster extract will require a lot of substrate in order to reach half of the Vmax. Portabella will need only a little bit of substrate to get to half of its Vmax, therefore it may be a good choice if there is a low amount of substrate. This means that the Oyster extract has a low enzyme affinity compared to the Portabella extract as it would take a higher substrate concentration to make it get half of its Vmax. Economically speaking, Portabella seems to be the more favorable enzyme when there is no glucosidal
From Table 1, it may be seen that an increase in the concentration of the inhibitor in portabella changes the Km from 0.33 to 1.6mM but presents no change in Vmax. It may also be seen from Table 1 that an increase in the concentration of the inhibitor in oyster increases the Km from 0.72 to 1.8mM and also presents a slight increase in Vmax from 3.82 to 4.07nmol/min. When no inhibition is added to the substrates, there is substantial difference between the Portabella and Oyster Km values, Portabella being 0.33mM and Oyster being 0.72mM. With 10mM of glucose, the difference still remains with Portabella being 1.60mM and Oyster being 1.80mM. Oyster mushroom also has a higher Vmax than Portabella with or without glucose inhibition, Oyster being 3.82nmol/min and Portabella 2.21nmol/min without inhibition and Oyster being 4.07nmol/min and Portabella 2.21nmol/min with 10mM of glucosidal inhibition. All the data stated above may be found illustrated as …show more content…
Because the Km value for the Oyster extract is significantly larger, this means that the Oyster extract will require a lot of substrate in order to reach half of the Vmax. Portabella will need only a little bit of substrate to get to half of its Vmax, therefore it may be a good choice if there is a low amount of substrate. This means that the Oyster extract has a low enzyme affinity compared to the Portabella extract as it would take a higher substrate concentration to make it get half of its Vmax. Economically speaking, Portabella seems to be the more favorable enzyme when there is no glucosidal