In one petri dish I used 15ml of pure distilled water (the typical environment of daphnia) as my control to look back on and compare to my experimental group my altered variables. In my three other dishes I started with 15 ml of pure water, 2-3 daphnia, and introduced 2 ml of harmful substance ( Prestone :antifreeze,Bayer Advanced: pesticide, and Malaboro :cigarette ash). After exposing the daphnia to these chemicals, I observed each petri dish and studied the change in behavior, appearance, and function every five minutes over a time span of a half an hour. I rated the change on a scale of 0 to 5. 1 being death, 3 being normal, and 5 being change in behavior and appearance. I averaged the rate for each experiment based on the daphnia’s behavior and appearance which typically reacted the same
In one petri dish I used 15ml of pure distilled water (the typical environment of daphnia) as my control to look back on and compare to my experimental group my altered variables. In my three other dishes I started with 15 ml of pure water, 2-3 daphnia, and introduced 2 ml of harmful substance ( Prestone :antifreeze,Bayer Advanced: pesticide, and Malaboro :cigarette ash). After exposing the daphnia to these chemicals, I observed each petri dish and studied the change in behavior, appearance, and function every five minutes over a time span of a half an hour. I rated the change on a scale of 0 to 5. 1 being death, 3 being normal, and 5 being change in behavior and appearance. I averaged the rate for each experiment based on the daphnia’s behavior and appearance which typically reacted the same