Escherichia Coli ( E. Coli Essay

1468 Words Nov 25th, 2016 6 Pages
Escherichia coli (E. coli) is one of the most widely studied and understood living organisms. It has been used for decades in furthering understanding of gene functionality and gene-protein mechanisms for prokaryotes and eukaryotes alike (Riley et al. 2006).
Prokaryotes, like E. coli, have their DNA in the form of a plasmid. A plasmid is a circular ring of DNA. The plasmid intended to transform the E. coli colonies for the purposes of this study was the pGLO plasmid. The pGLO plasmid contains three main genes: one for GFP (green fluorescent protein), one for ampicillin (an antibiotic) resistance, and one that codes for the sugar arabinose (Bassiri 2011). The process used to transform the E. coli bacteria with the pGLO plasmid was by means of a “heat shock.” The heat shock process is a form of genetic transformation by which an increase in temperature causes cell membranes to become porous allowing the influx of genetic information, such as that contained within the pGLO plasmid. The heat shock process has been shown to be an effective strategy for genetic transformation in virtually all eukaryotes (Gourse 2016). The heat shock was the form of genetic transformation chosen for this study due to the cost-effectiveness and ubiquitous nature of the heat shock response found in both prokaryotic and eukaryotic cells alike.
This study was carried out in order to see if E. coli is a competent bacteria cell able to take up a pGLO plasmid by means of heat shock genetic…

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