Epstein-Barr Virus is part of the herpesvirus family and was the first virus discovered to cause a human cancer. The virus has two stages of its life, the latent phase, which allows the virus to lie dormant within a cell and the host. The other stage is the lytic phase in which the virus reproduces and allows the virus to spread among cells. Haloperidol (HPD) aids in the initiation of the lytic cycle. The Haloperidol will be administered at varying concentrations and exposure times. Using quantitative polymerase chain reaction (qPCR) will monitor the amplification of the BZLF1 gene and myc genes. These genes are regulatory genes that code for transcription factors; the process of copying DNA nucleotides into a new strand of RNA. The
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It infects the majority of the world’s adult population (1) and establishes a lifelong dormant infection. The virus persists in the body throughout a person’s lifetime without expressing any symptoms. Epstein-Barr Virus is part of the herpesvirus family and was the first virus discovered to cause a human cancer. During infection EBV switches between two phases of its life cycle; the lytic and latent phase. The latent phase is dormant and the virus incorporates its DNA into the host cell’s DNA. While the lytic phase involves viral reproduction, where the virus uses the cell’s machinery to reproduce and replicate inside the cell. A gene reactivates in the virus causing it to switch from latency to lytic phase allow the virus to spread among cells and between hosts. There are different molecules that are being studied that reactivate and inhibit the virus. Valpromide (VPA) prevents the virus from entering the lytic cycle and reproducing in the cells, it is shown through the suppression of the BZLF1 gene (2,4). The BZLF1 gene is the gene that regulates and controls the reactivation of the lytic cycle. On the other hand, sodium butyrate (NaB) promotes the reactivation of the lytic cycle (4). Reactivation of the lytic cycle plays a role in the formation of cancer, when the virus starts to reproduce and multiply. Testing various drugs can help contribute to what makes the virus reactivates and …show more content…
Using the process of quantitative polymerase chain reaction (qPCR) we will be able to determine to what degree the Haloperidol (HPD) activated the BZLF1 and myc genes. The BZLF1 and myc are genes that regulate functions such as transcription factors. Quantitative polymerase chain reaction detects the nucleic acid amplification for these specific genes. Based on the quantity of the nucleic acid, it will determine magnitude the genes were expressed. Understanding the relationship between the Epstein-Barr Virus and different drugs will be one of the first steps in the ultimate goal of developing new approaches for treating cancers caused by
It infects the majority of the world’s adult population (1) and establishes a lifelong dormant infection. The virus persists in the body throughout a person’s lifetime without expressing any symptoms. Epstein-Barr Virus is part of the herpesvirus family and was the first virus discovered to cause a human cancer. During infection EBV switches between two phases of its life cycle; the lytic and latent phase. The latent phase is dormant and the virus incorporates its DNA into the host cell’s DNA. While the lytic phase involves viral reproduction, where the virus uses the cell’s machinery to reproduce and replicate inside the cell. A gene reactivates in the virus causing it to switch from latency to lytic phase allow the virus to spread among cells and between hosts. There are different molecules that are being studied that reactivate and inhibit the virus. Valpromide (VPA) prevents the virus from entering the lytic cycle and reproducing in the cells, it is shown through the suppression of the BZLF1 gene (2,4). The BZLF1 gene is the gene that regulates and controls the reactivation of the lytic cycle. On the other hand, sodium butyrate (NaB) promotes the reactivation of the lytic cycle (4). Reactivation of the lytic cycle plays a role in the formation of cancer, when the virus starts to reproduce and multiply. Testing various drugs can help contribute to what makes the virus reactivates and …show more content…
Using the process of quantitative polymerase chain reaction (qPCR) we will be able to determine to what degree the Haloperidol (HPD) activated the BZLF1 and myc genes. The BZLF1 and myc are genes that regulate functions such as transcription factors. Quantitative polymerase chain reaction detects the nucleic acid amplification for these specific genes. Based on the quantity of the nucleic acid, it will determine magnitude the genes were expressed. Understanding the relationship between the Epstein-Barr Virus and different drugs will be one of the first steps in the ultimate goal of developing new approaches for treating cancers caused by