Introduction Enzymes, which speed up a chemical reaction without being consumed or changing the products of the reaction, are very important because they control the cellular activity of all living organisms. Enzymes are also described as being “catalysts”. In biological systems, specific enzymes catalyze particular cellular reactions. Each enzyme has a unique 3-D shape, and this shape determines which reaction it catalyzes. However, in order for a chemical reaction to move forward, an energy barrier…
experiment, the effect of environmental conditions on enzyme functions was tested. The materials needed for the experiment was an amylase enzyme to lower the activation energy of the reactions, starch solutions of pH 3,4,5,6,7,and 8, these provided different concentrations to project various environmental conditions. In addition, Iodine solution, and a spectrophotometer were needed. Each cuvette contained a specific pH concentration. Then the enzyme extract was added to each pH concentration for a reaction…
effect the rate of enzymes were tested: concentration, temperature, and pH levels; to try and find the absorbance rate of all three factors of enzymes. Each one being measured by a spectrophotometer every twenty seconds for 120 seconds. Eleven test tubes were numbered from one to eleven as well as eleven cuvettes. Each cuvette received one drop of guaiacol, three drops of hydrogen peroxide, and 2.8 mL of pH 7 buffer. Each test tube received different contents to measure the enzyme activity. The test…