EXPERIMENT: The effect of an acidic fluid on the activity of an enzyme
INTRODUCTION: There are several factors that may influence the activity of an enzyme to include temperature and the pH levels. In order to better understand how the pH level affects the actions of an enzyme, the experiment will introduce different foods with acids and bases through mixture of direct contact.
OBJECTIVE: The objective of this experiment is to recognize how the introduction of an acid affects the activities of an enzyme.
MATERIALS REQUIRED: Meat, acidic foods, potatoes, alkaline foods, a scaled beaker, yeast, boiling tube, hydrogen peroxide, 100 milliliter graduated cylinder, and a dropper
HYPTHOSIS: If the pH level can affect the activity of an enzyme, then …show more content…
Utilizing an acidic food and an alkaline food will yield how the pH and the activity of the enzyme is effected on the acidic foods. With the catalase that is present in the yeast, the oxygen was released in bubble-form. By measuring the bubbles, the action of the enzymes can be easily seen. The experiment followed these steps:
1. One centimeter cubed meat added to the 100 milliliter graduated cylinder
2. Two centimeters-cubed of the source of the catalase enzyme, yeast, was added into the 100 milliliter graduated cylinder
3. Two milliliters of the hydrogen peroxide were put in a boiling tube
4. The graduated cylinder and the boiling tube were both placed into a water bath that had a temperature of 82 degrees Fahrenheit
5. Once they were at the same temperature, I poured the hydrogen peroxide into the graduated cylinder. At the same time, I noted the overall initial volume of the of fluid in the graduated cylinder
6. After waiting two minutes, I made note of the volume of the fluid in the graduated cylinder
7. I subtracted the initial volume from the final volume in order to determine the change in
INTRODUCTION: There are several factors that may influence the activity of an enzyme to include temperature and the pH levels. In order to better understand how the pH level affects the actions of an enzyme, the experiment will introduce different foods with acids and bases through mixture of direct contact.
OBJECTIVE: The objective of this experiment is to recognize how the introduction of an acid affects the activities of an enzyme.
MATERIALS REQUIRED: Meat, acidic foods, potatoes, alkaline foods, a scaled beaker, yeast, boiling tube, hydrogen peroxide, 100 milliliter graduated cylinder, and a dropper
HYPTHOSIS: If the pH level can affect the activity of an enzyme, then …show more content…
Utilizing an acidic food and an alkaline food will yield how the pH and the activity of the enzyme is effected on the acidic foods. With the catalase that is present in the yeast, the oxygen was released in bubble-form. By measuring the bubbles, the action of the enzymes can be easily seen. The experiment followed these steps:
1. One centimeter cubed meat added to the 100 milliliter graduated cylinder
2. Two centimeters-cubed of the source of the catalase enzyme, yeast, was added into the 100 milliliter graduated cylinder
3. Two milliliters of the hydrogen peroxide were put in a boiling tube
4. The graduated cylinder and the boiling tube were both placed into a water bath that had a temperature of 82 degrees Fahrenheit
5. Once they were at the same temperature, I poured the hydrogen peroxide into the graduated cylinder. At the same time, I noted the overall initial volume of the of fluid in the graduated cylinder
6. After waiting two minutes, I made note of the volume of the fluid in the graduated cylinder
7. I subtracted the initial volume from the final volume in order to determine the change in