In this experiment three different environmental conditions that effect the rate of enzymes were tested: concentration, temperature, and pH levels; to try and find the absorbance rate of all three factors of enzymes. Each one being measured by a spectrophotometer every twenty seconds for 120 seconds. Eleven test tubes were numbered from one to eleven as well as eleven cuvettes. Each cuvette received one drop of guaiacol, three drops of hydrogen peroxide, and 2.8 mL of pH 7 buffer. Each test tube received different contents to measure the enzyme activity. The test tubes were mixed in with the cuvettes, which would be the solutions we measured in the spectrophotometer. Cuvettes 2,3,4 varied in enzyme concentration, cuvettes 2,5,6,7 varied in
