Enzyme Catalysis Lab Essay

1178 Words Feb 13th, 2011 5 Pages

After reviewing the basics of enzymes and catalysis, we take a dive into the wonderful world of catalase. Beginning with establishing a base line of just how much hydrogen peroxide there is in 5.0mL of the reacted solution; to figuring out exactly how much actually reacted after 300 seconds of catalyzed reaction. Follow the experiment from the beginning steps right to the end as you see where the students went wrong, interpretation of the results, and great answers to work sheet questions. Now, dive into the amazing world of catalase catalysis.

Background Information:

Enzymes are catalytic proteins, meaning they speed up – but do not create – chemical reactions, without being used up or altered permanently in the
…show more content…
The third level of organization, tertiary structure, consists of irregular loops and folds which form as the protein folds and are held in place be disulfide bridges and hydrophobic interactions. Disulfide bridges are strong covalent bonds that occur between the sulfurs of various amino acids; hydrophobic interactions occur when large numbers of hydrophobic side chains come together and ‘stick’ to one another. Quaternary structure, the fourth and final level of protein organization, occurs only when multiple polypeptides come together in a single protein. This structure is the final overall three- dimensional shape of the protein.

As a result of four levels of organization, an enzyme has a very specific shape, which is called its conformation. Even more specific is the active site of the enzyme, where the actual catalysis occurs. The specific molecule or closely related molecules on which an enzyme functions is known as its substrate. Shape plays such an important role in enzymatic catalysis, that often even isomers of the substrate will be rejected. Once the substrate enters the active site, it may begin a process known as induced fit in which the enzyme perfectly conforms to the molecule to allow for more efficient catalysis.

Enzymes have specific environmental conditions at which they will function best. As a result, changes in environment can severely impact enzyme catalysis

Related Documents