The objective of this lab was to see how enzymes were used, use the scientific method, and how enzymes are affected by temperature.
Introduction
Proteins which constitute enzymes in their final form are macromolecules that make up large amounts of living organism which are called polymers which are made up of many different monomers. An enzyme is a biological catalyst that increases the rate of a reaction by lowering activation energy. Activation energy that the enzyme uses id an energy barrier that must be overcome before a chemical reaction can proceed (Lecture, 4/28). Enzymes are very specific and only work with one particular substance called a substrate molecule using an activation site, this is called the Lock and Key Theory (Lecture …show more content…
With the first test tube, 0 degrees, add 10 drops of the enzyme solution (the teacher will provide the enzyme). Pour the contents from test tube one into a clean 250mL Nalgene bottle and swirl it. Place the O2 Gas Sensor into the bottle making sure it is tight and nothing is able to leak out. After putting the sensor in the bottle, wait 30 seconds and then start collecting data. Data should be collected for 180 seconds. Once the 180 seconds are complete, remove the sensor from the bottle, rinse and dry the bottle. Make sure that the neck of the bottle is dry so that the O2 Gas Sensor does not get wet. With the sensor, preform a linear regression to calculate the rate of reaction. To do this, choose Curve Fit form the Analyze menu, then select Linear for the Fit equation. Record the value of m as the reaction rate in Table 1. Next, move on to the next test tubes. Use the directions above to complete the test tubes for 27 degrees, 37 degrees, and 55 degrees. Note: when it’s time to do the test tube for 55 degrees, the instructor will give you a special enzyme that has been heating. The results from my lab are as following; test tube 1, 0 degrees, had the lowest rate of oxygen at .029353, test tube 2, 23 degrees, had the highest rate of oxygen at .040048, test tube 3, 37 degrees had an oxygen production rate of .039904, and test tube 4, 55 degrees had an oxygen production rate of …show more content…
It says that once you hit room temperature, the “heat” you are adding to the enzyme is making it denature, or start to lose its shape and function. We know that once it loses its shape and function, that enzyme is “dead”and it can’t do what it is supposed to do anymore which means that our cells are not going to be functioning the way they are supposed to. Remember though, once an enzyme starts the denature process, it cannot be reversed. This is why we see the rate of oxygen production decreasing after we go over room temperature. As we were going through the lab, there were a couple things that we could have done differently. There were a couple problem we faced, but if we were to do it again we would be able to change that. One thing that went wrong was when we were trying to test the regular water bath test tube, 37 degrees, our O2 Gas Sensor wasn’t working correctly, so we had to go back and do it again. If I can remember correctly, when we went to do it again, I don’t think we waited the full ten minutes before we tested it. Also, I believe that on one of the test tubes, we added too many drops of the enzyme which could definitely affect the outcome of oxygen production. If we had to do this lab again, we would be a lot more careful about how many drops of the solutions and enzymes we added. Also,
Introduction
Proteins which constitute enzymes in their final form are macromolecules that make up large amounts of living organism which are called polymers which are made up of many different monomers. An enzyme is a biological catalyst that increases the rate of a reaction by lowering activation energy. Activation energy that the enzyme uses id an energy barrier that must be overcome before a chemical reaction can proceed (Lecture, 4/28). Enzymes are very specific and only work with one particular substance called a substrate molecule using an activation site, this is called the Lock and Key Theory (Lecture …show more content…
With the first test tube, 0 degrees, add 10 drops of the enzyme solution (the teacher will provide the enzyme). Pour the contents from test tube one into a clean 250mL Nalgene bottle and swirl it. Place the O2 Gas Sensor into the bottle making sure it is tight and nothing is able to leak out. After putting the sensor in the bottle, wait 30 seconds and then start collecting data. Data should be collected for 180 seconds. Once the 180 seconds are complete, remove the sensor from the bottle, rinse and dry the bottle. Make sure that the neck of the bottle is dry so that the O2 Gas Sensor does not get wet. With the sensor, preform a linear regression to calculate the rate of reaction. To do this, choose Curve Fit form the Analyze menu, then select Linear for the Fit equation. Record the value of m as the reaction rate in Table 1. Next, move on to the next test tubes. Use the directions above to complete the test tubes for 27 degrees, 37 degrees, and 55 degrees. Note: when it’s time to do the test tube for 55 degrees, the instructor will give you a special enzyme that has been heating. The results from my lab are as following; test tube 1, 0 degrees, had the lowest rate of oxygen at .029353, test tube 2, 23 degrees, had the highest rate of oxygen at .040048, test tube 3, 37 degrees had an oxygen production rate of .039904, and test tube 4, 55 degrees had an oxygen production rate of …show more content…
It says that once you hit room temperature, the “heat” you are adding to the enzyme is making it denature, or start to lose its shape and function. We know that once it loses its shape and function, that enzyme is “dead”and it can’t do what it is supposed to do anymore which means that our cells are not going to be functioning the way they are supposed to. Remember though, once an enzyme starts the denature process, it cannot be reversed. This is why we see the rate of oxygen production decreasing after we go over room temperature. As we were going through the lab, there were a couple things that we could have done differently. There were a couple problem we faced, but if we were to do it again we would be able to change that. One thing that went wrong was when we were trying to test the regular water bath test tube, 37 degrees, our O2 Gas Sensor wasn’t working correctly, so we had to go back and do it again. If I can remember correctly, when we went to do it again, I don’t think we waited the full ten minutes before we tested it. Also, I believe that on one of the test tubes, we added too many drops of the enzyme which could definitely affect the outcome of oxygen production. If we had to do this lab again, we would be a lot more careful about how many drops of the solutions and enzymes we added. Also,