Introduction The effect different temperatures have on the rate an enzyme works was tested by this lab. The concentration of the enzyme catalase in different types of cell was also tested. Enzymes are balls of protein that change a specific substrate into a product by contact at the active site. Enzymes are reusable and once they finish tuning one substrate into a product, it moves on to the next substrate it bumps into in just the right way. The enzyme catalase, which this lab focuses on, can be found in the tissues of plants and animals. Catalase is very important because it takes in hydrogen peroxide, which is toxic to our cells,as its substrate and breaks it into two non-toxic products that we consume every day, water and oxygen. Peroxide
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Each piece of liver, potato, and meat was contained by a test tube for the reaction to take place. An additional test tube to heat and cool the peroxide was needed, totaling 7 test tubes of the same size. Test tubes can't stand up by themselves, so they were held by a test tube rack. Because test tubes also can’t measure liquids, the peroxide was measured by a graduated cylinder. The ice, hot, and warm bathes were made in large beakers. At least 2 large beakers where needed. The peroxide to be used in the lab was contained by a smaller beaker. In order to avoid being burnt by the hot water, a test tube clamp was used to place the test tubes in the beaker of boiling water. Rather than touching the pieces of liver, forceps were used to place them into each test …show more content…
First, a match was lit by swiping it across the striking surface. Then, the wooden splint was lit by the lit match. When the splint began to glow orange, the flame on the splint was blown out. Immediately after being blown out the burnt end of the splint was held over the test tube where the reaction was taking place. The splint would be relit by oxygen, put out by carbon dioxide, or make a pop caused by hydrogen.A 0-5 rating scale was used to compare the reaction of catalase and hydrogen peroxide in different conditions. A 0 was no reaction, 1 was very little or slow reaction, and 5 was a very fast reaction. The rating scale also gave a quantitative value to a qualitative
Each piece of liver, potato, and meat was contained by a test tube for the reaction to take place. An additional test tube to heat and cool the peroxide was needed, totaling 7 test tubes of the same size. Test tubes can't stand up by themselves, so they were held by a test tube rack. Because test tubes also can’t measure liquids, the peroxide was measured by a graduated cylinder. The ice, hot, and warm bathes were made in large beakers. At least 2 large beakers where needed. The peroxide to be used in the lab was contained by a smaller beaker. In order to avoid being burnt by the hot water, a test tube clamp was used to place the test tubes in the beaker of boiling water. Rather than touching the pieces of liver, forceps were used to place them into each test …show more content…
First, a match was lit by swiping it across the striking surface. Then, the wooden splint was lit by the lit match. When the splint began to glow orange, the flame on the splint was blown out. Immediately after being blown out the burnt end of the splint was held over the test tube where the reaction was taking place. The splint would be relit by oxygen, put out by carbon dioxide, or make a pop caused by hydrogen.A 0-5 rating scale was used to compare the reaction of catalase and hydrogen peroxide in different conditions. A 0 was no reaction, 1 was very little or slow reaction, and 5 was a very fast reaction. The rating scale also gave a quantitative value to a qualitative