Enzyme Activity Lab Report

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Objective:
To understand the function of an enzyme when it undergoes different conditions such as temperature, PH, and concentration of the enzyme.

Introduction: In our lab “Enzyme Action” we were testing the enzyme reaction with different substrates. In our lab we tested 3 different enzymes and substrates to find out when they are at the highest potential. Enzymes are necessary for life because they keep our bodies chemical reactions at a normal pace. Enzymes have the ability to keep a human body at homoeostasis. In our lab we were finding when the enzyme is the most active while undergoing different temperature, PH, and concentrate.
Results:

In our first part of our experiment we tested the affect temperature on our enzymes and our substrates. When the enzymes were in a warmer environment they didn’t work as well as they did in the cooler environments. From our data we can see that the enzyme was least effective at 37 degrees and at 65 degrees. The temperature that it was most active was at 22 degrees. The time it takes to reach optimal effectiveness was around 180 seconds. They all started at 30 seconds around .1 to .15. When we reached 180 seconds the enzyme was slowing down and ranged from .35 to .65. The graph also shows that the experiment was prepared and performed very closely to instructions. Temperature had a great
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The graph does not show any kind of correlation at all. In this graph you can see that PH had a great impact on the enzymes effectiveness. With the higher the PH you can see that the enzyme was more of a base enzyme and substrate then a acidic one. With the graph not having any correlation is may show that the scientist may have made an error in the experiment. They could have got the data wrong with all the different test tubes. They also should do a repeat experiment to see if that was the right data. PH made a very large difference in the way the enzymes and substrates

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