In this experiment, the effects of environmental conditions on enzyme activity are explored. An enzyme is better known as an element produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. The environmental condition that was examined were the effects of temperature on enzyme activity. The raw materials that were used in this experiment were potato pile used to create catechol oxidase, catechol, and water which added as the solution. With Pipets and cubits used to measure and mix the solutions together. The instrument used to record the absorbency of the solutions was a spectrophotometer. The spectrophotometer is an instrument which measures the light of a specific wavelength which passes through
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In this trial. The effects of environmental conditions on enzyme activity were explored. The environmental situation that was observed was the effects ' temperature have on the enzyme samples. The aim of this study was to calculate the temperature effects on the reaction rate of catechol oxidase and catalyzed reactions. If the temperature in the solution altered or changed the enzyme in the sample then the alternative hypothesis will be accepted which states when temperature is present the enzyme will react considerably and the temperature will affect the enzyme reaction rate. If the alternative hypothesis is proven wrong the null hypothesis will be accepted which states when temperature, is present the enzyme will not affect and will continue catalyzing reactions at the same rate. Methods and Materials To start the experiment by one-half of a fresh potato will be needed to create the catechol oxidase. Catechol also known as pyrocatechol or dihydroxybenzene is an organic compound. This compound will be utilized in this experiment because it is known to speed up the reaction …show more content…
Adding one milliliter of water and one milliliter of catechol oxides to a cubit. This will be repeated for each cubit. Slowly dilute each sample down making the fourth sample the most diluted one. The fifth cubit sample will be used to blank the spectrometer machine at 420 absorbency maximum. After the spectrometer has been blanked begin adding the catechol. Add four-five drops of catechol to each cubit, one at a time, so the other solutions will not contaminate or affected by other properties. The first sample will be placed into the spectrophotometer and record its absorbed wavelength throughout a time period of ten minutes. Repeated this process four times, each time with a change in temperature. Results Depicted in figure 1 are the values that were collected throughout this experiment shown on a graph. This figure shows the data that was collected being compared to each other. This was significant to the study because it assisted in showing which sample had the highest absorbency when compared to the wavelength. Shown in figure 2 is the average rate that all the samples increased throughout the reaction
In this trial. The effects of environmental conditions on enzyme activity were explored. The environmental situation that was observed was the effects ' temperature have on the enzyme samples. The aim of this study was to calculate the temperature effects on the reaction rate of catechol oxidase and catalyzed reactions. If the temperature in the solution altered or changed the enzyme in the sample then the alternative hypothesis will be accepted which states when temperature is present the enzyme will react considerably and the temperature will affect the enzyme reaction rate. If the alternative hypothesis is proven wrong the null hypothesis will be accepted which states when temperature, is present the enzyme will not affect and will continue catalyzing reactions at the same rate. Methods and Materials To start the experiment by one-half of a fresh potato will be needed to create the catechol oxidase. Catechol also known as pyrocatechol or dihydroxybenzene is an organic compound. This compound will be utilized in this experiment because it is known to speed up the reaction …show more content…
Adding one milliliter of water and one milliliter of catechol oxides to a cubit. This will be repeated for each cubit. Slowly dilute each sample down making the fourth sample the most diluted one. The fifth cubit sample will be used to blank the spectrometer machine at 420 absorbency maximum. After the spectrometer has been blanked begin adding the catechol. Add four-five drops of catechol to each cubit, one at a time, so the other solutions will not contaminate or affected by other properties. The first sample will be placed into the spectrophotometer and record its absorbed wavelength throughout a time period of ten minutes. Repeated this process four times, each time with a change in temperature. Results Depicted in figure 1 are the values that were collected throughout this experiment shown on a graph. This figure shows the data that was collected being compared to each other. This was significant to the study because it assisted in showing which sample had the highest absorbency when compared to the wavelength. Shown in figure 2 is the average rate that all the samples increased throughout the reaction