Unknown Bacteria 228
Adisney Pino
Professor Dowding
Miami Dade College
Section T/R 7:05PM
Introduction Enterobacter aerogenes is rod-shaped bacteria that belongs in the family of Enterobacteriaceae. This rod-shaped bacterium is gram negative which makes it a facultative anaerobe which grew perfectly in 37-degree temperature. This species was motile and therefore it can synthesize an enzyme known as ornithine decarboxylase. This bacterium is prevalent in the intestines of animals, there presence in the intestines of animals makes it easier to get in the soil, water and sewage. In humans, this bacterium is known as an opportunistic pathogen which can cause multiple conditions which include, meningitis, bacteremia, pneumonia, …show more content…
Then, we looked at the Tributyrin agar and looked for a clearing/halo around the bacteria. After this procedure, we inoculated three urea broths and after storing it at our optimal temperature for 48 hours we brought it out and examined them for color change. After these tests had been run and observed we grabbed three nutrient gelatin stabs, and we inoculated two tubes, this time we stabbed the gelatin once with an inoculating needle and left one more nutrient stab for control purposes. The following lab we observed the gelatin, if it was solid or soft and watery. We also watched for any formation where we stabbed the gelatin. After these observations were done, we obtained a Simmons Citrate tubes, and made a zigzag pattern on the slant, lightly inoculating with a needle and incubated them all at our optimal temperature. The following lab we will watch the slant for any color change and growth. The following lab date we had a Phenylalanine Agar slant and inoculated heavily doing a zigzag motion and the lab class after that we added 2 or 3 drops of 12% ferric chloride solution to the tube and observed for color change. The reaction we were looking for should have happened
Adisney Pino
Professor Dowding
Miami Dade College
Section T/R 7:05PM
Introduction Enterobacter aerogenes is rod-shaped bacteria that belongs in the family of Enterobacteriaceae. This rod-shaped bacterium is gram negative which makes it a facultative anaerobe which grew perfectly in 37-degree temperature. This species was motile and therefore it can synthesize an enzyme known as ornithine decarboxylase. This bacterium is prevalent in the intestines of animals, there presence in the intestines of animals makes it easier to get in the soil, water and sewage. In humans, this bacterium is known as an opportunistic pathogen which can cause multiple conditions which include, meningitis, bacteremia, pneumonia, …show more content…
Then, we looked at the Tributyrin agar and looked for a clearing/halo around the bacteria. After this procedure, we inoculated three urea broths and after storing it at our optimal temperature for 48 hours we brought it out and examined them for color change. After these tests had been run and observed we grabbed three nutrient gelatin stabs, and we inoculated two tubes, this time we stabbed the gelatin once with an inoculating needle and left one more nutrient stab for control purposes. The following lab we observed the gelatin, if it was solid or soft and watery. We also watched for any formation where we stabbed the gelatin. After these observations were done, we obtained a Simmons Citrate tubes, and made a zigzag pattern on the slant, lightly inoculating with a needle and incubated them all at our optimal temperature. The following lab we will watch the slant for any color change and growth. The following lab date we had a Phenylalanine Agar slant and inoculated heavily doing a zigzag motion and the lab class after that we added 2 or 3 drops of 12% ferric chloride solution to the tube and observed for color change. The reaction we were looking for should have happened