Temporal analysis of gene expression in the embryonic murine Schwann cell lineage
1Anjali Balakrishnan, 2Morgan G. Stykel, 1,2Yacine Touahri, 2*Jeff Biernaskie, 1*Carol Schuurmans
Institutional Affiliations: 1Department of Biochemistry and Molecular Biology in the Cumming School of Medicine, 2Department of Comparative Biology and Experimental Medicine in the Faculty of Veterinary Medicine, both in the Alberta Children’s Hospital Research Institute and Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, CANADA
Abbreviated Title: Profiling embryonic Schwann cells
*Co-senior/co-corresponding authors:
Carol Schuurmans Jeff Biernaskie
HMRB, Room 250 HMRB, Room 402
Cumming School of Medicine Faculty of Veterinary …show more content…
SCPs follow a well-defined differentiation cascade, first giving rise to immature SCs and then to non-myelinating or myelinating SCs, the latter first progressing through a pro-myelinating stage. Multiple transcription factors regulate temporal identity transitions in the SC lineage, but how these factors act in a coordinated fashion remains poorly understood. To better understand cell fate decisions during SC genesis, we compared the temporal expression profiles of a panel of twelve transcription factors from embryonic day (E) 9.0, when neural crest cells (NCCs) are migrating, until E18.5, when pro-myelinating SCs populate the maturing spinal nerve. Two transcription factors, Sox9 and Sox10, were expressed from the onset of NCC migration and persisted until E18.5, allowing us to use Sox10 as a pan-glial marker for co-expression analyses. The earliest transcription factors co-expressed with Sox10 in ventrally migrating E9.0 NCCs were Sox9, Pax3, AP2 and Nfatc4, while E10.5 Sox10+ NCCs coalescing in the dorsal root ganglia expressed Sox9, Pax3, AP2 and Etv5. E12.5 SCPs continued to express Sox10, Sox9, AP2 and Pax3, as well as initiating Sox2 and Egr1 expression. E14.5 immature SCs were similar to E12.5 SCPs, except that they lost Pax3 expression. By
1Anjali Balakrishnan, 2Morgan G. Stykel, 1,2Yacine Touahri, 2*Jeff Biernaskie, 1*Carol Schuurmans
Institutional Affiliations: 1Department of Biochemistry and Molecular Biology in the Cumming School of Medicine, 2Department of Comparative Biology and Experimental Medicine in the Faculty of Veterinary Medicine, both in the Alberta Children’s Hospital Research Institute and Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, CANADA
Abbreviated Title: Profiling embryonic Schwann cells
*Co-senior/co-corresponding authors:
Carol Schuurmans Jeff Biernaskie
HMRB, Room 250 HMRB, Room 402
Cumming School of Medicine Faculty of Veterinary …show more content…
SCPs follow a well-defined differentiation cascade, first giving rise to immature SCs and then to non-myelinating or myelinating SCs, the latter first progressing through a pro-myelinating stage. Multiple transcription factors regulate temporal identity transitions in the SC lineage, but how these factors act in a coordinated fashion remains poorly understood. To better understand cell fate decisions during SC genesis, we compared the temporal expression profiles of a panel of twelve transcription factors from embryonic day (E) 9.0, when neural crest cells (NCCs) are migrating, until E18.5, when pro-myelinating SCs populate the maturing spinal nerve. Two transcription factors, Sox9 and Sox10, were expressed from the onset of NCC migration and persisted until E18.5, allowing us to use Sox10 as a pan-glial marker for co-expression analyses. The earliest transcription factors co-expressed with Sox10 in ventrally migrating E9.0 NCCs were Sox9, Pax3, AP2 and Nfatc4, while E10.5 Sox10+ NCCs coalescing in the dorsal root ganglia expressed Sox9, Pax3, AP2 and Etv5. E12.5 SCPs continued to express Sox10, Sox9, AP2 and Pax3, as well as initiating Sox2 and Egr1 expression. E14.5 immature SCs were similar to E12.5 SCPs, except that they lost Pax3 expression. By