The case group consist of 297 patient with approved chronic HBV infection. 220 male (aged 12-70, mean=34.93) and 77 female (aged 11-67, mean=33.75). The maximum and Minimum of the ages were 70 and 11 respectively, with total average of 34.62±12.61. The control group consist of total 333 healthy people aged 17-69, mean=38.4±10.7, 203 male, aged 17-69 and 130 female aged 18-69. The control group were clear according to immunologic, known infectious diseases and HBV serologic tests.
3.2. Genotyping results Eight genotypes for IL-1RN (VNTR) were observed in the present study which are represented in the Table 2. The allele frequency of IL1-RN gene reveals the dominancy of allele 1 and 2 (four and three copies of the 86-bp repeat respectively), in both cases and controls groups. Consequently, genotype one (A1/A1) was mostly observed with a frequency of 59.3% cases and 40.2% in control group. The genotype 6 (A2/A2) and 2 (A1/A2) are on the second and third rank of frequency with …show more content…
However the frequency of the genotype T/T and C/T which were almost in equilibrium in case group (34.7% and 28.3% respectively) were observed to be very different in healthy controls. The frequency of genotype T/T was dramatically increased to 55.6% and frequency of genotype C/T was decreased to 9.3%. This pattern of observed genotypes lead to increase of the allele T frequency controls (39.7% ) in comparison to C allele (60.3%) in the healthy. While in the chronic cases grope the frequency of alleles C and T were very closed together (51.1% and 49.9% respectively). This difference was detected as highly significant using Chi-square test (p<0.00001). The Hardy-Weinberg principle was in equilibrium state for both case and control groups. The frequencies of the alleles and genotypes are compared in table
3.2. Genotyping results Eight genotypes for IL-1RN (VNTR) were observed in the present study which are represented in the Table 2. The allele frequency of IL1-RN gene reveals the dominancy of allele 1 and 2 (four and three copies of the 86-bp repeat respectively), in both cases and controls groups. Consequently, genotype one (A1/A1) was mostly observed with a frequency of 59.3% cases and 40.2% in control group. The genotype 6 (A2/A2) and 2 (A1/A2) are on the second and third rank of frequency with …show more content…
However the frequency of the genotype T/T and C/T which were almost in equilibrium in case group (34.7% and 28.3% respectively) were observed to be very different in healthy controls. The frequency of genotype T/T was dramatically increased to 55.6% and frequency of genotype C/T was decreased to 9.3%. This pattern of observed genotypes lead to increase of the allele T frequency controls (39.7% ) in comparison to C allele (60.3%) in the healthy. While in the chronic cases grope the frequency of alleles C and T were very closed together (51.1% and 49.9% respectively). This difference was detected as highly significant using Chi-square test (p<0.00001). The Hardy-Weinberg principle was in equilibrium state for both case and control groups. The frequencies of the alleles and genotypes are compared in table