S. Cerevisiae Case Study

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A number of studies have been reported on response of S.cerevisiae during oxidative stress whereas, a very limited information is available regarding K. marxianus. This yeast may serve as a good model for the analysis of oxidative stress response because the fermentation metabolism in this yeast differs in a significant manner as compared to S.cerevisiae. Moreover recently it was reported that there is a huge difference between genes of respiratory, oxidative and carbohydrate metabolism among S.cerevisiae and K. lactis (a sister species of K. marxianus). Among the various stresses encountered by yeast K. marxianus during fermentation of cheese whey into ethanol, the major stresses include osmotic, oxidative, heat stress and ethanol inhibition. …show more content…
marxianus MTCC1389, S.cerevisiae MTCC170 and S. cerevisiae CEN.PK2 yeast with different concentrations of H2O2, at different growth phases (12, 24, 48 and 72 h) was determined by checking viability after the oxidant was added. All the yeast strains grew up with almost 100% of viability when no oxidant was present in the medium. Fig. 1A shows that in case of K.marxianus, treatment with 50 mM H2O2 was found lethal for exponentially growing cell and no survivability was observed in 12 h and 24 h cells. However, the impact of oxidant H2O2 was reduced during stationary growth phase (48 h and 72 h) and more than 20% cell viability was detected in 48 h and 72 h cells at 50 mM H2O2. The reason for the higher viability of stationary phase on exponentially growing cells may be because, the stationary phase cells were stressed by a lack of nutrients and build-up of toxic metabolites, and were set apart in ways that allow maintaining their viability for prolonged periods without further nutrients. Despite the viability of stationary phase cells at 50 mM H2O2 it should be noticed that the maximum cell viability was observed at 10 mM H2O2 and was significantly higher as compared to 20 mM H2O2 and 50 mM

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