Lipase Lab Report

1668 Words 7 Pages
Contents
Introduction 2
Aim 3
Hypothesis 3
Materials List 3
Safety Considerations 4
Hotplates 4
Potential Health Effects: 4
First Aid Measures: 4
Lipase Solution 4
Potential Health Effects: 4
First Aid Measures: 4
Sodium Carbonate 5
Potential Health Effects: 5
First Aid Measures: 5
Phenolphthalein 6
Potential Health Effects: 6
First Aid Measures: 6
Full Cream Milk 6
Test Tubes, Glass Rods and Measuring Beakers 7
Method 7
Results 8
Discussion 9
Conclusion 10
Appendix 10
Calculations: 13
Bibliography 13

Introduction
Enzymes are core components of reactions. Enzymes can be employed to hasten, or even delay reactions. Enzymes themselves are simply biological molecules (proteins) that affect a reaction as a catalyst. A catalyst is a chemical that induces a chemical reaction. Lipase is an enzyme that the human body uses to break down fats in foods so they can be absorbed in the intestines. Furthermore, lipase is essentially created in the pancreas but is also formed in the mouth and stomach (Encyclopedia Britannica, 2015). The substrate used will be milk; therefore milk will be used as a substance for the enzyme (lipase) to act upon it, which in-turn will create a reaction. The reaction between the enzyme (lipase) and the substrate (milk) will create a light pink solution, which will occur in the test tubes as the lipase solution breaks down the fats in the milk. How long this reaction will take place will depend purely on the temperature. The optimum
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Hypothesis
The anticipation is that as the temperature increases, so will the rate of enzyme reaction. However, as the temperature exceeds the optimum (37℃ to 45℃) the rate of reaction will decrease, as it will become denatured, furthermore, at temperatures below 10℃ to 15℃ the enzyme will become inactive. (Shi HZ, 2015).
Materials List Beakers 5x Test tubes

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